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題名 | 過敏性鼻炎患者中醫體質類型與基因及蛋白質表現之相關性研究=The Study of the Relationship between Gene and Protein Expression and the Chinese Medical Constitutional Types in the Patients of Allergic Rhinitis |
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作者 | 徐松錕; | 書刊名 | 中醫藥年報 |
卷期 | 24:2 民95.10 |
頁次 | 頁149-192 |
專輯 | 中醫藥基因體及免疫學研究 |
分類號 | 413.52 |
關鍵詞 | 過敏性鼻炎; 中醫; 體質; 臨床試驗; 二維電泳膠蛋白質分析; Allergic rhinitis; Chinese medicine; Constitution; 2D-gel protein analytical; Clinical trial; |
語文 | 中文(Chinese) |
中文摘要 | 中醫的診斷利用人體在疾病的表現狀況,將人的體質分成寒、熱;虛、實;表、裡等等類別,利用屬性不同之中藥將不平衡的身體調至平衡以治療疾病。數千年來,體質特性與相關疾病間的關係及其治療方法均已詳細的紀錄在中醫的典籍裡,不同中藥的藥性與其對人體的影響也有詳細記載。人類基因序列已公布,許多分子生物的技術與資訊提供很好的方法來分析細胞的整體表現,突破以往以一個基因為主的研究方式。這種以大量基因表現分佈的研究方式與中醫的診斷方式,利用個體的特徵來診斷疾病有相似之處。 本研究延續92年的計畫,以2D-gel蛋白質分析的技術研究中醫寒性或熱性體質患者的蛋白質表現,以及服用不同屬性寒熱中藥之影響,進而探討蛋白質表現之相關基因。作為尋找體質「寒熱」類型相關之分子依據。研究採用臨床試驗模式,164名經西醫確診為過敏性鼻炎患者,隨機分為二組分別服用寒性或熱性中藥方二個月,在服藥前後分別接受中醫體質分型、西醫臨床及實驗室評估,其中包括血清蛋白質分析。以了解寒熱不同體質患者之基因相關蛋白質表現、寒熱不同中藥方對不同體質患者基因相關蛋白質表現之影響。 研究之結果顯示:性別、時序及藥物對體質表現會產生影響,服用體質調節藥方藥後均可改善過敏性鼻炎患者臨床表現。在病人服用中藥前後的蛋白質比對中,我們發現有二個點的差異與服用寒與熱藥物有正相關,經蛋白質定序後,發現為血紅蛋白α與ß,部分服用寒藥的病患會增加,服用熱藥者會減少。另外有許多蛋白質的差異,部分已完成定序,而在體質類型變化,蛋白表現變化、藥物作用時間之關聯仍應再做分析。 |
英文摘要 | In Chinese medicine, the patients' constitutional types are classified according to their manifestation in disease circumstances, and different types of Chinese herbs are used to treat the patients by correcting the unbalanced status of body. In recent years, instead of studying single gene, many molecular biological information and techniques provide many tools to analyze the whole genome expression of the cell. These methods can be used to investigate the protein and mRNA expression in large quantities at the same time and shows a gene or protein expression character of each individual, which is similar to the Chinese medical diagnostic method that they make use of the characteristics of individual for diagnosis. This grant is continuation of grant of year 92. In order to find out the related genes for the “cold” and “hot” constitutional types in the future, we started with the 2D-gel protein analytical methods to study the protein expresssion of the two groups of patients, which belongs the “hot” and “cold” constitutional types respectively. And we also study the effects of the “cold” and “hot” types of Chinese herbs in this study. We performed the clinical trial and 164 patients were included in our study after being diagnosed as “Allergic rhinitis” by professional ENT doctor. The patients were grouped randomly into 2 groups, and each took the “cold” or “hot” Chinese medical remedy for 2 months respectively. The patients were examined for Chinese medical constitutional typing and western medical clinical and laboratory evaluation before and after taking the remedies. The results indicates the constitutions were affected by the gender, seasons and types of medicine. The patients' signs and symptoms were improved after taking the Chinese medical remedies. The expression of the protein before and after taking the remedies was positively correlated with the effects of the “hot” and “cold” remedy in 2 proteins which were identified to be hemoglobin α and ß. Many protein expression differences were detected and their protein sequences were identifyied. The correlation among the changes of the constitutional types, protein expression and the effects of the remedy intervention period still need further analysis. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。