查詢結果分析
來源資料
相關文獻
- 應用專一高效能之人類白血球功能檢驗法評估具抗發炎及免疫增強作用之中草藥並以動物試驗確認藥效
- Fas and CD45RO Antigen Expression on Peripheral Blood Mononuclear Cells in Patients with Ankylosing Spondylitis: A Comparison with Autoimmune Diseases
- Evaluation of Chinese Herbs that Affect the Cell-mediated Immunity(3)
- Spleen Rupture in Infectious Mononucleosis: Report of One Case
- The Effect of "Chi-Han (Hot Nature)" Chinese Herbs on the Secretion of Il-1β and Tnf-α by Mononuclear Cells
- Inhibition of 2-Aminofluorene Acetylations by Vitamin C in Monouclear Leukocytes of Spregue-Dawley Rats
- Interleukin-I Production from Monocyte Induced by Urate Crystals
- Acute Monoblastic Leukemia in a Child Following Chemotherapy for Neuroblastoma
- 黃芩素及其他異黃酮類成分對大鼠肝中乙醯轉移酶活性之影響
- Naloxone Suppresses Cytokines Production in LPS-stimulated Human Monocytic THP-1 Cells
頁籤選單縮合
題名 | 應用專一高效能之人類白血球功能檢驗法評估具抗發炎及免疫增強作用之中草藥並以動物試驗確認藥效=Evaluation and Mechanism (s) Studies of the Principles or Extracts Isolated from Traditional Chinese Medicine for the Anti-inflammatory and Immuno-modulating Potential by Specific and High Performance Cellular and Animal Model |
---|---|
作者姓名(中文) | 沈郁強; | 書刊名 | 中醫藥年報 |
卷期 | 24:1 民95.10 |
頁次 | 頁75-105 |
專輯 | 中醫藥療效評估之研究 |
分類號 | 414.5 |
關鍵詞 | 嗜中性球; 單核球; 活性氧屬自由基; 黏著分子; 黃芩素; 黃連解毒湯; 普濟消毒飲; Neutrophil; Mononuclear cell; Inflammation; Reactive oxygen species; ROS; Baicalein; |
語文 | 中文(Chinese) |
中文摘要 | 本計畫以人類白血球建立壹高效能之抗發炎及抗氧化細胞評估方法,快速驗證具抗發炎之中草藥,可進一步研究藥物標的或機轉,或再以動物試驗確認藥效。方法是以分離之人類嗜中性球(PMN)或單核球(MNC)探討單複方中(草)藥可否抑制體外人類PMN及MNC之活化,包括製造活性氧屬自由基(reactive oxygen species; ROS)、細胞緊密黏著(firm adhesion)、黏著分子(Mac-1)表現、細胞鈣離子內流(calcium influex)等。評估之中草藥包括清燥濕之黃芩、黃連、黃柏,抗病毒之金銀花、板藍根,含黃芩之複方包括:黃連解毒湯、龍膽瀉肝、普濟消毒飲及不含黃芩之玉女煎、知柏地黃丸等共10種中藥濃縮製劑。我們分離之PMN及MNC純度分別可達95%及85%以上。於抗ROS之實驗中,不管是對接受體依賴型(fMLP-induced)或是蛋白激酶C依賴型(PMA-mediated)之PMN及MNC活化,皆以黃芩、普濟消毒飲、黃連解毒湯三方之抑制能力最強,IC[9274]約為5-10 µg/ml。正對照標準品baicalein(黃芩素)之IC[9274]約為7-11 µg/ml。對照複方成份之普濟消毒飲、黃連解毒湯接含黃芩,因此黃芩、普濟消毒飲、黃連解毒湯三方抗ROS之作用應與baicalein含量正相關。清熱滋陰之玉女煎、知柏地黃丸之IC[9274]較高為20-30 µg/ml。板藍根(10-15 µg/ml)對MNC之ROS製造反而有促進之作用,因此對提升刺激免疫發炎反應有促進作用。金銀花則無此免疫促進作用。進一步分析PMN之緊密黏著分子Mac-1表現,發現確實可以反應上述黃芩、普濟消毒飲、黃連解毒湯三方之抗發炎作用,它們在50 µg/ml都可顯著抑制(50-80%)PMN之緊密黏著及黏著分子Mac-1表現。以Fura2及Fura3研究這10種藥方對細胞鈣離子內流之影響,結果並不一致。因為藥方多為黃色,因Fura3較不受顏色影響,因此依據Fura3之結果我們認為這些藥方會單獨提升細胞鈣離子內流;黃芩及板藍根可抑制發炎刺激物fMLP誘發之細胞鈣離子內流。結論我們成功建立系列人類白血球發炎篩選藥物模式包括活性氧屬自由基(ROS)、細胞緊密黏著、黏著分子(Mac-1)表現、活化鈣離子之細胞內流。比較三種ROS偵測方式(冷光、螢光、可見光)以冷光方式最為敏感,適合研究抗發炎作用。應用於分析臨床方劑,研究對清熱燥濕藥之分析顯示它們對人類白血球發炎自由基及緊密黏著、黏著分子表現有較一致之抑制效果,並與已發表之結果吻合(baicalein/黃芩)。另外篩選得本所分離抗發炎新成份包括乳藤、牛樟芝及一些野生磨菇類成份約10餘種。 |
英文摘要 | In the present project we set up series of in vitro specific and high-performance functional assays by human leukocyte as acute inflammatory cellular models for the elucidation of anti-inflammatory drugs from natural products and traditional Chinese medicine (TCM). These models include reactive oxygen species (ROS) production, firm adhesion, adhesion molecule (e.g., Mac-1) up-expression, and intracellular calcium mobilization. All of which were important inflammatory responses by human leukocytes. Based on these models, we could either screen/select potential herb or components easily, or study the mechanisms of action in mediating the anti-inflammatory effect by the TCM or natural products. First of all, we have successfully purified human neurophils (PMN) and mononuclear cells (MNC) from peripheral whole blood with purity more than 95% and 85%, respectively. Six TCM remedies, either single or multiple component(s), with heat-clearing and damp-drying (清熱燥濕) activity were selected including Radix Scutellariae (黃芩), Rhizoma Coptidis (黃連), Cortex Phellodendri (黃柏), Pu-chi-siou-du-in (普濟消毒飲), Hung-lian-gia-du-tan (黃連解毒湯), and Long-dan-shae-gan-tan (龍膽瀉肝湯). Two herbal drugs with anti-virus activity--Radix Isatidis (板藍根) and Lonicerae Flos (金銀花), and two TCM remedies for nourishing Yin and falling fire (滋陰降火)--Yu-nue-gen (玉女煎) and Zu-po-di-huang-wan (知柏地黃丸) were also included as reference drugs. For the anti-ROS activity, Radix Scutellariae (黃芩), Pu-chi-siou-du-in (普濟消毒飲), and Hung-lian-gia-du-tan (黃連解毒湯) were all effective in the inhibition of fMLP (receptor-mediated) and PMA (PKC-activated) -induced ROS production both in PMN and MNC with 50% inhibition concentration (IC[9274]) around 5-10 µg/ml. Baicalein, a known active standard from Radix Scutellariae as a positive control, also significantly prevents fMLP- and PMA-induced ROS production by PMN and MNC with IC[9274] around 7-11 µg/ml. Since Radix Scutellariae (黃芩), Pu-chi-siou-du-in (普濟消毒飲), and Hung-lian-gia-du-tan (黃連解毒湯) all contain baicalein, it is possible that baicalein plays a dominant role in the inhibition of ROS production by human leukocytes. Actually, we have previously reported that baicalein could interfaere leukocyte activity through a G-protein dependent calcium influx pathway. The remedies for nourishing Yin and falling fire-Yu-nue-gen (玉女煎) and Zu-po-di-huang-wan (知柏地黃丸) -were relative less effective than the above mentioned heat- clearing and damp-drying drugs with IC[9274] more than 20 µg/ml. Notably, the anti- virus drug-Radix Isatidis (板藍根), but not Lonicerae Flos (金銀花), could enhance the ROS production by MNC illustrating that the immuno-enhancing activity of Radix Isatidis may correlate with its ROS promoting activity by MNC. Furthermore, anti-firm adhension and anti-Mac-1 up-expression studies also reveals that Radix Scutellariae (黃芩), Pu-chi-chiou-du-in (普濟消毒飲), and Hung-lian-gia-du-tan (黃連解毒湯) all exhibit promising effect in these two models at 50 µg/ml examined. For intracellular calcium signaling, we did not find good correlation of the anti-inflammatory effect by these effective drugs in fMLP- induced intracellular calcium mobilization model, either estimated by Fura2- or by Fura3-loaded PMN. In conclusion, we have successfully set up series of specific inflammatory models for the evaluation of TCM memedies and herbal or natural drugs. Based on these models, especially the ROS production detected by a chemi-luminescence-enhanced method, we could properly predict the anti-inflammatory potential of TCM remedies. The anti-inflammatory activity of the heat-clearing and damp- drying (清熱燥濕) drugs could be explained by their active component-baicalein, which has been reported to be an effective anti-inflammatory drug in our previous study. We also find 10 more new fractions/components from Antrodia camphorata (牛樟芝) and Parsonsia laevigata (乳藤) by these methods. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。