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題 名 | 放線桿菌胸膜肺炎不活化菌苗之研發=Development of an Actinobacillus Pleuropneumoniae Inactivated Vaccine |
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作 者 | 張惟茗; 林敬覆; 吳義興; 蕭終融; | 書刊名 | 行政院農業委員會家畜衛生試驗所研究報告 |
卷 期 | 39 民92 |
頁 次 | 頁43-47 |
分類號 | 437.657、437.657 |
關鍵詞 | 胸膜肺炎放射桿菌; 毒素; 疫苗; Actinobacillus pleuropneumoniae; Toxin; Vaccine; |
語 文 | 中文(Chinese) |
中文摘要 | 為進行放線桿菌胸膜肺炎不活化菌苗之研發,種菌之選殖共測試包括21株第1血清型分離株及分屬第1、2、5及11血清型之4株標準株等菌株共25株,所有分離菌株均先經生化試驗及血清學同定確認。菌株之篩選主要針對ApxI細胞毒素之分泌量及菌落型態進行篩選。ApxI細胞毒素之檢測則以酵素免疫斑點法(使用抗ApxI毒素單源抗體檢測)及溶血力價測定等兩種方式進行。在25株菌株中,21株為平滑型菌落,2株為粗糙型,2株為黏液型。以酵素免疫斑點法檢測菌株之ApxI毒素分泌,結果力價之分布由陰性至1:256,差異相當大。菌株溶血力價之分布則由陰性至1:32。不同菌株在酵素免疫斑點法力價或溶血力價與培養於血液培養基所產生之溶血圈大小一致。最後選殖之種菌為第1血清型分離株,菌落為黏液型,酵素免疫斑點法力價及溶血力價分別為1:256及1:32。以豬胸膜肺炎放線桿菌第1血清型種菌進行大量培養所得之菌液,再利用硫酸銨濃縮後檢測其毒素含量減少低於10%,但是毒素活性卻大幅降低,減少超過90%。將菌液不活化後添加商品化油質佐劑之試製菌苗所進行之特性試驗,純粹試驗、無菌試驗及安全試驗等之結果均符合動物用藥品檢驗標準之規定。試製菌苗所進行之效力試驗,計算試製菌苗之防禦指數為1.41,高於標準。 |
英文摘要 | A selection of a seed strain for development of Actinobacillus pleuropneumoniae (Ap) vaccine was made from 4 type strains and 21 field strains. Among 25 strains, 21 strains were smooth type, 2 were rough type and 2 were mucus type according colony morphology. The criteria of the selection were based on the secretion of ApxI toxin, since it was one of the major factors of pathogenesis, and on colony morphology. The detection of ApxI toxin was made by immnoblotting test and hemolytic test. The distribution of titer by immnoblotting test and hemolytic test were from negative to 1 : 256 and from negative to 1 : 32, respectively. It was coordinated with the hemolytic zone appeared in blood agar cultured. The seed strain selected was a field isolate in 1997 with titer of 1 : 256 and 1 : 32 tested by above tests.The cytotoxin contained in culture supernatant of Actinobacillus pleuropneumoniae serotype 1 was concentrated by salting out of ammonium sulfate. The amounts of cytotoxin were lost less than 10% after concentration process when measured by immuno-blotting assay. However, the biological activity of cytotoxin was decreased greatly for more then 90% when detected by hemolytic test. The physical properties, purity, sterility, and safety of prepared inactivated vaccine with addition of a commercialized oil adjuvant were all fit the national corresponding regulation. The evaluation of protective efficacy of prepared bacterin was done by mice protection test. The protective index was 1.41 and the inactivated vaccine was proved to be effective. |
本系統中英文摘要資訊取自各篇刊載內容。