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題名 | 茶白紋羽病及其病原性測試之探討=Study on the Pathogenicity of Dematophora Necatrix Isolated from Camellia Sinensis |
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作者姓名(中文) | 林秀穗; 謝煥儒; | 書刊名 | 臺灣茶業研究彙報 |
卷期 | 25 民95.11 |
頁次 | 頁17-29 |
分類號 | 434.818 |
關鍵詞 | 茶; 茶樹根部病害; 白紋羽病; 病原性測試; Camellia sinensis; Tea root disease; Dematophora necatrix; Pathogenicity test; |
語文 | 中文(Chinese) |
中文摘要 | 於南投之茶園發現有立枯茶樹,採其根腐檢體並分離出白紋羽菌。其於馬鈴薯葡萄糖培養基上之年輕菌落呈白色絨毛狀,老化菌落有黑色素產生,鏡檢發現其部分菌絲明顯具有隔膜處呈梨形膨大特徵,故認定其為白紋羽菌之無性世代(Dematophora necatrix Hartig);將其進行人工接種後可從罹病之實驗株中再度分離白紋羽菌完成柯霍式法則。另外,春季及秋季時於塑膠溫室內以青心烏龍茶苗為材料,在室溫,潮濕環境下進行各種接種源與寄主之組合,以尋求最適之病原性測試條件;在時間與成本雙重考量下,茶白紋羽菌之病原性測試最適條件為:使用25℃黑暗中培養1個月之燕麥粒接種源20g來進行1年生之青心烏龍仟插茶苗之人工接種,此組合下人工接種約1個月後可開始產生急性或慢性萎凋之二種茶白紋羽病徵,而2至3個月內適於進行病害評估。 |
英文摘要 | White root rot pathogen was isolated from root rot sample of Camellia sinensis (L.) O. Kuntze gathered in one dieback disease from a suffering tea garden in Nantou. The young colony of pathogen on PDA appeared while and velvety, while colony aged melanin was produced. Since some aged hypha had the characteristic pear-shape swellings near septa, the pathogen was identified as Dematophora necatrix Hartig. Pathogenicity tests were performed with a variety of conditions in a plastic greenhouse using Chin Shin Oolong tea cuttings as the material for artificial inoculations under room temperature and a humid environment in the spring and autumn of 2004, separately. Dematophora necatrix could be re-isolated from the diseased tea cuttings to fulfill the Koch's postulates. As time and costs are considered, the optimum condition for pathogenicity test is 20g of 1M old oat grain as inoculum with 1 year-old chin Shin Oolong tea cuttings as the host, incubated in a plastic greenhouse under an ambient environment. Both the acute and chronic symptoms of white root rot were initialized around 1month after the artificial inoculations and the disease development was suitable for evaluation during 2 to 3 months. |
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