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頁籤選單縮合
題名 | 臺灣地區青心烏龍品種外表型及AFLP標記變異之研究=The Assessment of the Morphological Variation and AFLP Marker Analysis in the Chin-Shin-Oolong Tea Cultivar in Taiwan |
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作 者 | 蔡憲宗; 蔡依真; 廖文如; 張清寬; 王裕文; | 書刊名 | 臺灣茶業研究彙報 |
卷期 | 23 民93.11 |
頁次 | 頁21-30 |
分類號 | 434.815 |
關鍵詞 | 核酸分子標記; 青心烏龍; 茶樹; 遺傳分析; 擴增片段長度多型性分析; 遺傳歧異度; DNA marker; Chin-shin Oolong; Tea Genetic analysis; AFLP analysis; Genetic diversity; |
語文 | 中文(Chinese) |
中文摘要 | 茶樹為多年生作物,以往皆是利用農藝性狀差異來區分不同的品種,但是這些都易受環境及栽培方式影響而改變,而呈現出一個連續性的變異,用傳統的方法來分類族群是有困難的,且可能無法反映出真實的遺傳相似度。青心烏龍為臺灣地區最重要的栽培品種,栽培面積超過50%,據傳為林鳳池氏於咸豐五年(1855)自大陸福建引入青心烏龍茶苗,種植於凍頂山,並於1910年由平鎮茶業試驗支所,從既存之地方品種中進行選種,由總督府命名並加以推廣種植。青心烏龍在不同地區調查其農藝性狀有明顯差異,但無法確認是環境影響造成其外表型的差異,或是本身遺傳物質不同造成的。 本研究利用增殖片段長度多型性(AFLP)分子片段標記來調查臺灣個地區所栽培之青心烏龍品種DNA變異情形。比較來自幾個主要茶區的30個不同茶園樣品,使用9組引子進行多型性分析,調查結果顯示不同地區之青心烏龍其核酸序列具有變異,30個樣品的平均遺傳相似度達92%,差異最大的樣品其相似度只有56%,提供了外表性狀差異性的遺傳上證據。 |
英文摘要 | Tea tree is a perennial species propagated mainly through vegetative methods. The morphological characteristics are subject to environment and cultivation practices and thus variations have been observed. The distinguishable morphological differences have been documented in literatures and yet they failed to offer genetic explanation. Chin-Shin-Oolong is the most important tea cultivar in Taiwan and accounts for more than 50% of the acreage of the tea plantation. The variety was originated from Mainland China and introduced by Lin, Fong-Tsu in the form of a plantlet in 1855. It was selected among with many other lines in 1910 in the Pin-Chun Tea Experiment Branch Station and formally released to the public. This research was intended to address the morphological variation using DNA marker technology to provide a genetic explanation. Thirty leave samples from the primary Chin Sin Oolong plantations around the island were collected and the DNA polymorphism among the samples was analyzed with AFLP marker system. The result revealed an average of 8% of DNA polymorphism among the 30 samples, which is greater than the amount of variation caused by mutation alone in general. There was a sample that had much greater diversity with the rest of samples and reached the magnitude of 44% of DNA difference. The morphological difference of this sample is less than the one revealed by DNA fragments. The result from this sample provides evidence of the genetic contribution of the variation. The DNA difference estimated for this sample was assumed to have been compromised by other factors during the expression of morphological traits. It was concluded the morphological difference among the Chin Sin Oolong tea samples in this study has genetic bais and the source has yet to be identified. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。