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題 名 | Genetic Diversity among Flue-cured Tobacco (Nicotiana Tabacum L.) Revealed by Amplified Fragment Length Polymorphism=應用擴增片段長度多型性研究烤煙遺傳歧異度 |
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作 者 | 張漢堯; 劉小珍; 李桐森; 楊宇明; | 書刊名 | Botanical Studies |
卷 期 | 47:3 民95.07 |
頁 次 | 頁223-229 |
分類號 | 434.824 |
關鍵詞 | 擴增片段長度多型性分析; 烤煙; 遺傳歧異度; 非加權對組算術平均法; 分子變異分析; AFLP analysis; AMOVA; Flue-cured tobacco; Genetic diversity; UPGMA; |
語 文 | 英文(English) |
中文摘要 | 烤煙是世界上最重要的商品作物之一。遺傳歧異度研究可對不同材料的遺傳變異水準進行估測,並可用於種質保存、品種保護,和烤煙品種的改良。對51個烤煙品種的擴增片段長度多型性 (AFLP) 分析產生了總數為1479條的明確的DNA片段。用非加權對組算術平均法(UPGMA)的聚類分析表明烤煙品種可劃分為美國型或中國型。中國型品種可進一步分為四個亞群,美國型品種也可進一步劃分為兩個亞群。品種間的平均成對遺傳距離為0.167,變化範圍為從0.024到0.267。AMOVA分析表明55.76%的變異產生于不同起源地的品種間,44.24%的變異則是來源於相同起源地的品種間。全部51個材料的遺傳分化指數Fst為0.177,每個材料對其餘材料的平均遺傳分化指數Fst變化範圍在0.152到0.238之間。本研究的帶型所揭示的資訊表明,當今普遍種植的烤煙種質的遺傳歧異度很小,有必要探取長期有效的措施以保護烤煙種質資源,進一步的雜交也應當在遺傳關係較遠的品種間進行。 |
英文摘要 | Flue-cured tobacco (Nicotiana tabacum L.) is one of the most important commercial crops in the world. Genetic diversity studies provide estimates on the level of genetic variation among diverse materials that can be used in germplasm management, varietal protection, and flue-cured tobacco improvement. Amplified fragment length polymorphism (AFLP) analysis of 51 flue-cured tobacco cultivars produced a total of 1479 unambiguous DNA fragments. Cluster analyses using the unweighted pair group method with arithmetic mean (UPGMA) showed that the cultivars could be grouped into American or Chinese types, with the Chinese types being further clustered into four subgroups and American ones into two subgroups. The average pairwise genetic distance was 0.167 and ranged from 0.024 to 0.267. AMOVA analysis showed that 55.76% of genetic variation came from cultivars having different origins and 44.24% from cultivars having the same origin. The overall average Fst of the 51 accessions was 0.177. Mean Fst of each accession against the rest ranged from 0.152 to 0.238. The findings of this study revealed that the present day commonly grown flue-cured tobacco germplasm has narrow genetic diversity among the cultivars, necessitating a sustained effort to preserve flue-cured tobacco germplasm resources. Further crosses should be made only with genetically distant varieties. |
本系統中英文摘要資訊取自各篇刊載內容。