頁籤選單縮合
題名 | Biologically-Feasible Screening Strategy for Optimal Decolorization Strain to Diazo Evercion Red H-E7B=對雙偶氮染料反應性紅色141生物上可行之最佳脫色菌株篩選策略 |
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作者 | 陳博彥; Chen, Bor-yann; |
期刊 | Journal of the Chinese Institute of Chemical Engineers |
出版日期 | 20060300 |
卷期 | 37:2 民95.03 |
頁次 | 頁117-124 |
分類號 | 445.463 |
語文 | eng |
關鍵詞 | 雙偶氮染料; 脫色菌; Reactive azo dye; Bacterial decolorization; Azoreductase; Toxicity; Growth association; |
中文摘要 | 本研究首次嘗試以產程生長相關性之觀點來系統性篩選出針對目標雙偶氮反應性紅色141號染料(RR141)處理之最佳脫色菌株。依據發酵工程上對生物產程之分類來進行比序,得到三種被評估之脫色菌生物活性大小依序為E. coli UVT1>P. luteola>>E. coli UV68。原染料RR141及其衍生中間物之相下生物毒性可能是造成低生物脫色之導因。對P. luteola而言,氧氣造成對染料脫色之抑制特性,乃源於脫色過程需仰賴對氧氣敏感之偶氮還原?之表達以進行非生長相關脫色作用之緣故。相對地,大腸桿菌UVT1可能因含有除偶氮還原酵素以外,較具多樣非專一化之脫色潛能,而造成較P. luteola為高之脫色效果。相較前述二者可推論,UVT1之高脫色效能可能是因為RR141及其中間脫色產物對菌體不具明顯毒性所致。但是,可能由於原本對偶氮染料具相當容忍阻抗能力之DNA基因片段被激發產生突變改質之故,UV68對染料之脫色活性則完全被抑制無法表達。 |
英文摘要 | This study provides a first attempt form a growth-association perspective to put forward, in general terms and explanation, plausible selection on optimal decolorize to a model diazo dye Evercion Red H-E7B (C.I. reactive red 141; RR141). According to Gaden’s classification scheme of fermentation, the bioactivity series of three decoloizers used in this study is Escherichia coli UTV1 > Pseudomonas luteola>>Escherichia coli UV68. The relative toxicity among the parent dye RR141 and its derived intermediates is likely the leading cause of low biodecolorization performance. For P. luteola, the oxygen-repression characteristics on decolorization of RR141 is due to the obligatory requirement of oxygen-sensitive azoreductase expression to precede non-growth-associated decoloization. In contrast, as E. coli UTV1 might contain a more diverse decoloization capability besides axoreductase, it proceeded color removal in relatively higher rates than P. luteola. Compared to other strains, the highest color removal performance of UVT1 might be due to negligible toxicity of intermediary products and dye RR141. However, perhaps owing to induced mutation occurred on ”DNA domains” originally dealing with bacterial tolerance to azo dyes, decoloization capability of UV68 to RR151 was found to be completely repressed. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。