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| 題 名 | Effects of Citrus Grandis Peels on Cyclosporin Concentration and Immune Responses in Mice=小鼠併服化橘紅對環孢靈體內濃度及免疫反應之影響 |
|---|---|
| 作 者 | 方世華; 侯鈺琪; 徐素蘭; 李珮端; | 書刊名 | 藥物食品分析 |
| 卷 期 | 14:2 2006.06[民95.06] |
| 頁 次 | 頁166-173+220 |
| 分類號 | 414.51 |
| 關鍵詞 | 化橘紅; 環孢靈; 免疫反應; 輔助型第一型/第二型T細胞細胞激素; Citrus grandis; Cyclosporin; Immune response; Th1/Th2 cytokines; |
| 語 文 | 英文(English) |
| 中文摘要 | 先前的研究顯示,豬於併服化橘紅水煎劑後,血液中環抱靈濃度顯著提高且引起急毒性。在本研究中,我們主要探討小鼠併服化橘紅水煎劑與環孢靈後,對於環孢靈分布在淋巴組織及非淋巴組織中濃度的影響;同時,分析小鼠免疫細胞功能的變化。結果顯示小鼠併服化橘紅水煎劑 (3g/kg) 及環孢靈 (10mg/kg) 兩週後,環孢靈濃度在血液、肝臟、腎臘及脾臟中分別下降了37%、45%、46%及51%,且濃度下降程度與併服化橘紅之劑量成正相關。另一方面,併服化橘紅逆轉了單服環抱靈所造成吞噬細胞吞噬能力的降低;另外,環抱靈對活化吞噬細胞產生一氧化氮的降低與輔助型第一型T細胞的細胞激素分泌的抑制,也因併服化橘紅而藥效下降。本研究結果顯示,併服化橘紅顯著降低環孢靈之體內濃度及業效,因此,臨床上化橘紅與環孢靈併用時,應嚴密監控其安全性。 |
| 英文摘要 | A previous study indicated that the coadministration of decoction of Citri Grandis Pericarpium (CGP), the peels of Citrus grandis (Rutaceae), significantly increased the blood level of cyclosporin (CsA) and resulted in acute intoxication of pigs. In this study, we aimed to measure the effects of CGP on the distribution of CsA in both lymphoid and non-lymphoid tissues as well as on the immune cell function in mice. Our results showed that coadministration of 3 g/kg CGP with CsA (Neoral®, 10 mg/kg) significantly decreased the concentrations of CsA in blood, liver, kidney and spleen by 37 %, 45 %, 46 % and 51 %, respectively, after a two week treatment. The decrease in CsA level correlated positively with the CGP dose. Pharmadynamically, coadministration of CGP restored the phagocytosis activity in blood decreased by CsA. Moreover, the nitric oxide production of activated macrophages and helper T cell type 1 (Th1) cytokines production suppressed by CsA were also reversed by the coadministration of CGP. In conclusion, coadministration of CGP significantly decreased the systemic exposure of CsA and resulted in higher macrophage and Th1 type activities than giving CsA alone in mice. Therefore, the combined use of CGP with CsA requires close monitoring clinically. |
本系統中英文摘要資訊取自各篇刊載內容。