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題 名 | Molecular Pattern and Antimicrobial Susceptibility of Methicillin-resistant Staphylococcus Aureus Isolates at a Teaching Hospital in Northern Taiwan=分析MRSA藥物敏感測試及分子生物學研究:北臺灣一教學醫院的經驗 |
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作 者 | 戴佩雯; 黃政華; 凌慶東; 黃仰仰; | 書刊名 | 微免與感染雜誌 |
卷 期 | 39:3 民95.06 |
頁 次 | 頁225-230 |
分類號 | 414.83 |
關鍵詞 | 藥物敏感測試; 分子生物學; Methicillin抗藥性金黃色葡萄球菌; Bacterial typing techniques; Epidemiology; Methicillin resistance; Microbial sensitivity tests; Staphylococcus aureus; |
語 文 | 英文(English) |
英文摘要 | Background and Purpose: Methicillin-resistant Staphylococcus aureus (MRSA) infection has progressively increased worldwide. Knowledge of the specific epidemiological pattern of isolates at individual hospitals is important. Methods: MRSA bacteremia was diagnosed in a total of 68 patients from January 2002 through December 2003, stratified for drug susceptibility and molecular pattern (staphylococcal cassette chromosome mec element [SCCmec] typing and genotypes). Results: SCCmec-A-positive isolates were found on polymerase chain reaction in 58 patients. The most frequent SCCmec types were III (40 cases) of which less than 5% were susceptible to other beta-lactam antibiotics and most were health care-associated, followed by SCCmec type IV (15 cases), that were demonstrated to be community-acquired. SCCmec type IV MRSA isolates were more likely to be susceptible to ciprofloxacin (93.3%), gentamicin (46.7%) and trimethoprim-sulfamethoxazole (93.3%) than type III isolates. All MRSA isolates were susceptible to glycopeptides and vancomycin (minimum inhibitory concentrations <2 μg/mL). Pulsed-field gel electrophoresis with SmaI digestion was used to fingerprint these isolates. A total of 9 genotypes with 26 type-subtypes were identified. Genotype A was the most frequent (9 subtypes) indicating that it is epidemic in this hospital. Conclusion: After analysis, SCCmec typing could be used to predict drug susceptibility. Specific clones of S. aureus are circulating in hospital and communities in Taiwan. |
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