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題名 | 臺灣水牛精液性狀之季節性變化=Seasonal Changes of Semen Characteristics in Taiwan Water Buffaloes (Bubalus Bubalis) |
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作者 | 魏良原; 劉炳燦; 賈玉祥; | 書刊名 | 畜產研究 |
卷期 | 38:4 民94.12 |
頁次 | 頁293-303 |
分類號 | 437.399 |
關鍵詞 | 臺灣水牛; 精液性狀; 季節性; Taiwan water buffalo; Semen characteristics; Seasonal changes; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗旨在建立臺灣水牛精液性狀之季節性變化與資料,作為冷陳精液製作之參考與製作季節選擇之依據。圈飼於花蓮地區(北緯23° 59’,東經121° 36’)之水牛3頭,以假陰道收集精液樣本,每週一回,每回收集兩次,間隔30分鐘,試驗為期一年。結果顯示,試驗期間,其精液性狀依次為精液量: 2.0±0.5Ml、精子濃度: 1.4±0.3×109 sperms/mL、泳動性評分: 4.6±0.4、精子活動力: 82.2±6.0%、精子畸形率: 7.0±1.5%、死精子率: 8.8±3.2%、pH值: 6.6±0.1。秋、冬季之精子沔度均顯著高於春、夏季(分別為1.5±0.3×109, 1.5±0.3×109 sperms/mL vs. 1.2±0.4×109, 1.3±0.2×107 sperms/mL, P<0.05)。冬季之精子活動力達全年之最高值(87.5±4.6%),並顯著(P<0.05)優於春(82.0±4.2%)、秋(78.8±8.1%)與夏季(82.1±3.5%)。夏季之死精子率為最高值(10.3±4.2%),亦顯著(P<0.05)高於其餘季節。而精液量、精子泳動性評分、精子畸形率和pH值等測量值,於各季節間均無顯著差異。間隔二次之精液收集,其精液性狀之各項測量值間,除射精量以第一次射精顯著較第二次多(2.1±0.6 vs. 1.9±0.4 Ml, P<0.05)之外,其餘性狀皆無顯著差異。試驗結果顯示,臺灣水牛精液以冬季之精子活動力和精子濃度等精液性狀為最佳,可於該季節進行精液冷凍保存作業。 |
英文摘要 | The objective of this experiment was to investigate the characteristics and seasonal changes of semen of Taiwan water buffaloes for determining a proper season for the frozen semen preparation. A total of three Taiwan water buffalo bulls kept in confinement in Hualien (23° 59,N, 121° 36,E) were used in the study for measurement of semen characteristics. Semen samples of 2 ejaculations were collected at 30-minute intervals with artificial vagina weekly throughout the year. Results of the semen characteristics were volume 2.0±0.5 mL, concentration 1.4±0.3×109/mL, mass activity score 4.6±0.4, motility 82.2±6.0%, percentage of abnormal spermatozoa 7.0±1.5%, percentage of dead spermatozoa 8.8±3.2% and pH 6.6±0.1, respectively. The average sperm concentrations in autumn and winter were significantly higher than in spring and summer (1.5±0.3×109, 1.5±0.3×109 vs. 1.2±0.4×109, 1.3±0.2×109 sperms/mL, P<0.05). The sperm motility in winter (85.7±4.6%) was significantly (P<0.05) higher than in spring (82.0±4.2%), autumn (78.8±8.1%) and summer (82.1±3.5%). The percentage of dead spermatozoa in summer (10.3±4.2%) was significantly (P<0.05) higher than the other seasons. There were no differences in sermen volume, mass activity score, percentage of abnormal spermatozoa and pH value between season. The semen volume was significantly (P<0.05) higher in 1st ejaculation than in 2nd (2.1±0.6 vs. 1.9±0.4 mL)ejaculation. The semen characteristics between 1st and 2nd ejaculation did not have any difference from each other. In conclusion, the sperm concentration and motility for Taiwan water buffalo bulls in winter were better than in summer season. Therefore, the semen collected in winter was more suitable for frozen semen preparation. |
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