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頁籤選單縮合
題名 | 生長調節物質對於大蒜培殖體生長之影響=Effect of Growth Regulators on the Growth of Garlic Explants |
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作者 | 張淨環; 廖松淵; Chang, Ching-huan; Liaw, Song-iuan; |
期刊 | 作物、環境與生物資訊 |
出版日期 | 20051200 |
卷期 | 2:4 民94.12 |
頁次 | 頁287-295 |
分類號 | 435.222 |
語文 | chi |
關鍵詞 | 培殖體; 起始培養基; 種原保存; SADH; Succinic acid-2,2-dimethyl hydrazide; MH; Maleic acid hydrazide; CCC; 2-chloroethyl-trimethylammonium chloride; Explants; Initial MS medium; Germplasm conservation; |
中文摘要 | 摘要 本研究以TA350 及TA613 二個大蒜種 原的培殖體為材料,將其培養在僅含30、 80 及100 gL-1 蔗糖的MS起始培養基(initial MS medium)中。經過3 個月後,再分別移 植至蔗糖濃度為30、80、100 gL-1 及分別含 1、10 及50 ppm SADH (succinic acid-2,2- dimethyl hydrazide) 或MH (maleic acid hydrazide)或CCC (2-chloroethyl-trimethylammonium chloride)的MS 培養基中,以 期能降低植株生長速率且不影響存活率的 條件下,篩檢出最適當延長繼代培養的條 件,以達到大蒜中期種原保存的目標。由研 究發現,TA350 及TA613 大蒜培殖體培養 在30 gL-1 蔗糖配合50 ppm SADH 或1 ppm MH 或50 ppm CCC 之處理組,植株 生長緩慢且具有較高的成活率。 |
英文摘要 | ABSTRACT The study was aimed to determine the best combination of sucrose concentration and growth regulator of MS medium for reducing the growth and giving the longest cycle of time per subculture. Two accessions of garlic explants grown in vitro were used in the experiment, namely, TA350 and TA613. The explants were cultured in the initial MS medium which contains 30, 80 and 100 gL-1 sucrose for 3 months, then transplanted to the 3 concentrations (30, 80, and 100 gL-1) of sucrose together with different concentrations (1, 10, and 50 ppm) of SADH (succinic acid-2,2-dimethyl hydrazide), MH (maleic acid hydrazide) and CCC (2-chloroethyl-trimethylammonium chloride), to determine the best combination for the germplasm conservation of garlic in vitro. The results indicated that the plantlets grown in 30 gL-1 sucrose together with 50 ppm SADH or 1 ppm MH or 50 ppm CCC showed reduced growth rate in vitro and high survival rate after transplantation. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。