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題名 | 國家同步輻射研究中心高效能蛋白質結晶學設施之發展現況=Current Status of Crystallography Beamlines and End Stations at NSRRC |
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作者 | 趙俊雄; 簡玉成; | 書刊名 | 科儀新知 |
卷期 | 27:4=150 民95.02 |
頁次 | 頁44-49 |
專輯 | 結構基因體學專題 |
分類號 | 368.6 |
關鍵詞 | 蛋白質結晶學; 生物分子; 同步輻射光; |
語文 | 中文(Chinese) |
中文摘要 | 白質結學是鑑定生物分子三空間結構的最有力工具之一,配合同步輻射光的高亮度及能量可調性,可以大幅提昇生物分子的結構鑑定速度,足以滿足基因體學及藥物設計等需要大量分子結構資料等研的需求。為此,因家同步輻射研究中心特別興建兩座高效能蛋白質結晶學光束線,其中一座為針對未知蛋白質結構解析用途,能量範圍從6.5至19仟電子伏特(keV)之多谺長異常繞射(MAD)光束線,另一座為供晶體篩選、藥物設計以及高解析度結構分析用,能量為12至14仟電子伏特之單色光(mono)光束線。使用最成熟可靠的光束線技術,配備最先進的面積偵測器系統、高速高效能的計算及網路環境、自動化的樣本裝卸系統,及友善的數據收集與控制系統,MAD光束線一年約可收集800組MAD數據,單色光光束線一年約可收集1200組高解析度數據。 |
英文摘要 | Protein crystallography is one of the most powerful and dominant tools to determine the three-dimensional structures of biological macromolecules today. In order to increase the rate of crystal structure determination, the highly brilliant and easily tunable synchrotron radiation (SR) X-ray source is essential to meet the needs of abound structural information for studying structural genomics and structural-based drug designs. To satisfy these requirements, the Natioanl Synchrotron Radiation Research Center (NSRRC) has constructed two dedicated Synchrotron Radiation Protein Crystallography Facility (SPXF) high-throughput beamlines for structural genomics research. One of the beamlines will be a multi-wavelength anomalous diffraction (MAD) beamline with energy tenability from 6.5 keV to 19 keV for unknown structure determinations, and the other will be a monochromatic (MONO) beamline with energy from 12 keV to 14keV for crystal screening, drug design and high-resolution structure studies. The well-devleoped light source technique, advanced area detectors, high-efficient computing and network environments,automatic crystal sample changing and centering, and user-friendly data collection and control stystems will produce 800 MAD data set per year for MAD beamline and 1200 useful data sets per year for mono beamline. . |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。