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題 名 | 番茄表面沙門氏菌及腸桿菌之檢測=Detection of Salmonella and Enterobacter Species on Tomato Surface |
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作 者 | 郭亭君; 邱采新; 王怡婷; 黃文哲; | 書刊名 | 農林學報 |
卷 期 | 54:2 民94.06 |
頁 次 | 頁93-100 |
分類號 | 445.981 |
關鍵詞 | 番茄; 沙門氏菌; 阪崎腸桿菌; Tomato; Salmonella; Enterobacter sakazakii; |
語 文 | 中文(Chinese) |
中文摘要 | 沙門氏菌一向被視為是主要存在於動物來源之肉類及蛋類食品中的食品病原菌,但近年來的食品中毒案例顯示,番茄、香瓜、果及柳橙等蔬菜及水果中也可能存在有沙門氏菌。本研究之目的即在於檢測市售番茄樣品中沙門氏菌可能之污染及分佈情形。於民國93年四月至六月期間由臺中市八個行政區域共採集32個聖女小番茄樣品,分別使用UP broth (Universal pre-enrichment broth)與Lactose broth沖洗表面所得到的洗液,先進行增殖培養24小時後,利用選擇性培養基(BS、HE及XLD agar)篩選出疑似沙門氏菌菌落,再分別使用Vitek identification system 及PCR(使用引子組INVA-1, INVA-2)進行細菌菌種鑑定。結果顯示,由32個市售番茄樣品表面並未離出任何沙門氏菌,但是在6個樣品中分離出Enterobacter sakazakii,其檢出率為18.8%。 |
英文摘要 | Salmonella species have been regarded as important foodborne pathgens mainly come from the contaminated animal-sourced egg, poultry and meat food products. More recently, salmonellosis has been linked to tomatoes, seed sprouts, cantaloupe, apple juice, and orange juice. This study was conducted aiming to detect the possible presence of Salmonella on the surface of tomato. Thirty two small tomato samples were collected from the eight districts of Taichung City during March to June 2004. The whole tomato samples were first rinsed with UP broth (Universal preenrichment broth) and Lactose broth in the plastic sampling bags, respectively. The rinse solution were pre-enrichmented at 35℃ for 24h, and then were inoculated onto Salmonlla selective agar plates, including BS、HE and XLD agar. The Salmonella-like colonies were picked up from the selective agar plates and identified by the Vitek identification system or PCR analysis with primers INVA-1 and INVA-2. The results showed that no Salmonella species were isolated and detected from the tomato samples. However, Enterobacter sakazakii was detected in 6 of the 32 tested samples with a diction rate of 18.8%. |
本系統中英文摘要資訊取自各篇刊載內容。