查詢結果分析
相關文獻
- The rDNA Sequence Analysis of Three Dendrobium Species
- 藉由分析核糖體核酸內轉錄間隔區探討臺灣原生石斛蘭之親緣關係
- Internal Transcribed Spacer Sequence Based Identification and Phylogenie Relationship of Herba Dendrobii
- 以核內的內轉錄區間序列探討臺灣馬兜鈴屬之親緣關係
- 臺灣疫病菌Phytophthora melonis, P. drechsleri及P. cryptogea之形態、生理特性及ITS序列之比較
- Phylogenic Relationships in the Drosophila nasuta Species Group (Diptera: Drosophilidae): A RAPD Approach
- 以PCR之普遍性引子增殖混合酵母菌體的核醣體RNA基因之靈敏度
- PCR-Mediated Detection of Ustilago Esculenta in Wateroat (Zizania Latifolia) by Ribosomal Internal Transcribed Spacer Sequences
- 杉木、香杉、柳杉及臺灣杉種間雜交性質之初步研究
- 利用trnL intron序列分析低等金縷梅類植物分子親緣
頁籤選單縮合
題 名 | The rDNA Sequence Analysis of Three Dendrobium Species=以核醣體去氧核糖核酸之核苷酸序列分析三個石斛品種 |
---|---|
作 者 | 鄭可大; 羅淑芳; 李鎮宇; 陳忠川; 蔡新聲; | 書刊名 | 藥物食品分析 |
卷 期 | 12:4 2004.12[民93.12] |
頁 次 | 頁367-369+374 |
分類號 | 414.32 |
關鍵詞 | 核醣體去氧核糖核酸; 石斛; 親緣關係; 內轉錄區; Dendrobium; Phylogeny; rDNA; ITS; |
語 文 | 英文(English) |
中文摘要 | 正確而精準的分子鑑定基源是避免藥用植物誤用的必要步驟。石餅藥材自古即為著名的治眼疾中藥材;然而石斛藥材的種類繁多,品種紊亂,再加上加工處理,導致外形辨認不易。本研究收集三個石斛品種:銅皮、黃花及金釵石斛,分析部分核醣體去氧核糖核酸核苷酸序列,建立rDNA序列資料庫,並分析其歧異度,暸解其親緣關係,作為今後鑑別的參考。以聚合酶連鎖反應擴增部分核醣體去氧核糖核酸區域之ITS 1、5.8S及ITS 2產物,銅皮石斛的長度為632個核苷酸,黃花石斛的長度為627個核苷酸,而金釵石斛的長度為628個核苷酸,其品種間之序列相似度從91%至95%不等。該結果將有助於石斛品種的鑑定。 |
英文摘要 | Positive molecular identification of medicinal plants is necessary to their misuse. Dendrobii Caulis is a famous Chinese herbal medicine traditionally used in ophthalmology. However, the identification of the species based on morphology is difficult. In this study, three Dendrobium species were collected and differentiated by the sequences of partial rDNA (ribosomal DNA) sections which contain the ITS 1, 5.8S, and ITS 2 regions. The Dendrobium species used in this reseach included Dendrobium tosaense, Dendrobium officinale, and Dendrobium moniliforme. The length of PCR products are 632 nt for D. tosaense, 627 nt for D. officinale, and 628 nt for D. moniliform, respectively. The similarities of the rDNA region between the species pairs shown range from 91% to 95%. The results we present here may be helpful in identifying other species of Dendrobium. |
本系統中英文摘要資訊取自各篇刊載內容。