頁籤選單縮合
題名 | 滲調處理促進結球萵苣種子發芽時endo-β-mannanase之活性=Priming Enhances endo-β-mannanase Activity in Lettuce (Lactuca sativa L.) Seeds |
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作者姓名(中文) | 宋妤; 江怡睿; | 書刊名 | 中國園藝 |
卷期 | 50:3 2004.09[民93.09] |
頁次 | 頁295-306 |
分類號 | 435.252 |
關鍵詞 | 萵苣種子; 發芽率; 滲調; 甘露醣酵素; Lettuce seed; Germination percentage; Priming; Mannanase; |
語文 | 中文(Chinese) |
中文摘要 | ‘Dark Green Boston’萵苣滲調種子於27℃較未滲調者提高28.8%發芽率,發芽整齊度與平均發芽天數均縮短。‘Everglad’萵苣滲調種子於33℃時較未滲調種子提高46.1%發芽率,增加發芽整齊度,達顯著性差異。於33℃‘達豐’萵苣滲調種子發芽率較未滲調種子提高47.6%,發芽整齊度、平均發芽天數差異不顯著。利用Gel-diffusion測定種子胚根尖端珠孔區及側邊胚乳之endo-β-mannanase活性於二發芽溫度下浸潤時,以胚根尖端珠孔區之活性較高,且滲調種子活性較未滲調者高。‘Dark Green Boston’萵苣滲調種子於20℃時產生與endo-β-mannanase為標識之相同位置編號2、3、4之蛋白質,浸潤於27℃使未滲調種子產生少量編號1及3蛋白質。 |
英文摘要 | At 27℃, the germination rate of primed ‘Dark Green Boston’ seeds was 28.8% greater than that of unprimed seeds. In addition, priming decreased the GT90-10 and the mean number of days to germination (MDG). At 33℃, priming increased the germination of ‘Everglade’ seeds by 46.1% and shortened GT90-10 by 0.4 days. At 33℃, priming increased the germination of ‘Mesa 659’ seeds by 47.6%, but it had no significant effect on GT90-10 and MDG. During lettuce seed germination, the endo-β-mannanase activity was measured with a gel-diffusion assay. In the primed lettuce seeds of all the three cultivars germinating at high or low temperatures, the endo-β-mannanase activity was higher in the micropylar region than in lateral regions. In addition, activity was higher in primed seeds than in unprimed ones. Based on two-dimensional gel electrophoresis fluorographs, primed ‘Dark Green Boston’ seeds imbibed at 20℃ produced protein No. 2, 3 and 4. Primed seeds imbibed at 27℃ made unprimed seeds produce spots of protein No. 1 and 3.‘’‘’ |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。