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題名 | 人體關節軟骨細胞分離與培養=Isolation and Cultivation of Human Articular Chondrocytes |
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作者姓名(中文) | 楊佩玉; 胡誕寧; 古鳴洲; 朱家宏; 陳輝星; 黃馨儀; 黃明和; | 書刊名 | 秀傳醫學雜誌 |
卷期 | 5:1/2 民93.04 |
頁次 | 頁61-65 |
分類號 | 415.132 |
關鍵詞 | 軟骨細胞; 細胞培養; 鹼性纖維母細胞生長因子; Chondrocyte; Cell culture; Basic fibroblast growth factor; |
語文 | 中文(Chinese) |
中文摘要 | 本文報告對一例因膝部骨關節炎切除的關節軟骨所作的成年人關節軟骨細胞的分離與培養。應用trypsin-collagenase消化法分離細胞。用加有10%胎牛血清、glutamine與gentamicin的F12培養液進行培養。軟骨细胞在體外生長良好,在10個月內傳代15次,細胞數增加為26.562倍,總分裂次數達15次。免疫細胞化學研究(抗S-100抗體)指示這些細胞培養為純軟骨細胞培養,本項研究顯示可用本文敘述之方法建立人類關節軟骨細胞培養。軟骨細胞培養可用於在體外研究關節軟骨細胞之生理及病理,並可用於自體軟骨細胞培養以治療關節軟骨缺損。 |
英文摘要 | Adult human articular chondrocytes were isolated from surgically excised auricular cartilage from a patient suffering from osteoarthritis of the knee. Cells were isolated by the trypsin-collagenase digestion method and cultured with F12 medium supplemented with 10% fetal bovine serum, glutamine and gentamicin. Cultured chondrocytes grew well. They were able to be passaged for 15 generations and revealed a 26.562-fold increase of cell numbers over a 10 month period (cumulative population doublings=15 times). An immunocytochemical study using anti-S-100 antibodies demonstrated that the culture was a pure cell culture of chondrocytes. The present study indicates that the cell cultures of adult human articular chondrocytes can be established by these methods. Cultured chondrocytes provide an in vitro model system for studying the physiology and pathology of human articular chondrocytes and may be used for autologous transplantation of chondrocytes to treat articular cartilage defects. |
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