查詢結果分析
相關文獻
- Effects of Salinity and pH on the Adherence and Virulence of Vibrio Cholerae O139
- Effect of Salinity on Egg Development of Black Porgy Acanthopagrus Schlegeli
- 猴頭菇以液體浸漬培養產製水溶性多醣類之探討
- Physical and Bio-Optical In-Situ Measurement Study on the Sewage Ocean Outfall Plume
- 環境因子對荔枝露疫病菌菌絲生長之影響
- 三種氫氧化鈣製劑對根尖周圍pH值之影響
- 以鹽度與溫度控制Chlorella Minutissima淡水與海水株綠球藻之長鏈不飽和脂肪酸產量及成份
- 2,3,5-Triphenyltetrazolium Reduction in the Viability Assay of Ulva fasciata (Chlorophyta) in Response to Salinity Stress
- Fluid Inclusions of the Lead-Zinc Quartz Veins, Chinmienshan, Toucheng
- Reduced Toxicity of Cu and Zn to Mangrove Seedlings (Kandelia Candel (L.) Druce.) in Saline Environments
頁籤選單縮合
題名 | Effects of Salinity and pH on the Adherence and Virulence of Vibrio Cholerae O139=鹽度及酸鹼度對霍亂弧菌O139吸附作用的影響 |
---|---|
作者 | 宋宏紅; 張建國; 藍世峰; | 書刊名 | 藥物食品分析 |
卷期 | 12:1 2004.03[民93.03] |
頁次 | 頁68-73+100 |
分類號 | 414.83 |
關鍵詞 | 鹽度; 酸鹼值; 霍亂弧菌O139; 吸附力; 毒力; Vibrio cholerae O139; Salinity; PH; Adherence; Virulence; |
語文 | 英文(English) |
中文摘要 | 霍亂弧菌感染人體引起霍亂的第一步必須能吸附小腸並進行拓殖 (colonization) 然而,吸附作用受生長環境中許多生理化學因子的影響。本研究探討在不同鹽度與酸鹼度下生長,霍亂弧菌O139的吸附力與毒力之變化。相較於在對照組培養基 (0.5% NaCl及pH7 0) 的培養,O139菌株於不同鹽度及酸鹼度培養後,其對HeLa細胞株的吸附率分別於0%及5%NaCl下,明顯降低56% (p=0.0007) 與69% (p=0.0008) 於pH6及pH5.5下,吸附率分別降低至91% (P=0.016) 和82% (p=0.006) 。經連續繼代培養後,其吸附力並無顯著變化 (P=0182) 若將第十代的細菌接種回對照組培養基中,其吸附力回升至與對照組相同。進行小鼠餵食及攻擊實驗,測定吸附力對O139菌株致病力的影響,結果顯示在0% NaCl、5% NaCl及pH5.5培養下,O139菌株對小鼠的存活率分別為50%、60%及20%。由這些結果推測,鹽度改變對菌株的吸附力及毒力造成的是可逆性抑制作用,且此影響強於酸鹼值的影響效應。 |
英文摘要 | Vibrio cholerae must first adhere to and colonize the human small intestine in order to cause cholera and this adherence can be affected by several physicochemical factors. The purpose of this study was to examine the adherence and virulence of V. cholerae O139 that was grown in experimental broth media with different salinities and pH values. After the bacterial cells were incubated separately in these media, the adherence of V. cholerae O139 to HeLa cells was examined. By comparison with bacteria grown in a control medium with 0.5% NaCl and pH 7.0, the percentages of adhesion of bacteria grown in media with 0% and 5% NaCl were found to decline significantly by 56% (P = 0.0007) and 69% (P = 0.0008) respectively, and those in media with pH 6 and 5.5 decreased to 91% (P = 0.016) and 82% (P = 0.006) respectively. After the bacterial cells were sub-cultured in the experimental media serially, no difference in adherence was observed between the first, the fifth and the tenth generations (P = 0.182). However, after the tenth generation, when the treated bacterial cells were re-incubated in a control medium, the level of adherence was found to become similar to that of the control bacteria. In order to determine the effect of adherence on the pathogenicity of V. cholerae O139, mice were challenged with a double dose of the LD50 of the test strains incubated in 0% NaCl, 5% NaCl, and pH 5.5, and the survival of rates were at 50%, 60% and 20%, respectively. These results suggest that changes of salinity can cause a reversible inhibition effect on either adhesion or virulence of V. cholerae O139, and those effects resulting from changes in salinity are stronger than those of different pH values. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。