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題 名 | Identification of VP7 Serotypes of Human Rotaviruses by Enzyme-Linked Immunosorbent Assay and Reverse Transcription-Polymerase Chain Reaction=以酶連免疫試驗及反轉錄-聚合酶連鎖反應鑑定人類輪狀病毒VP7血清型 |
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作 者 | 李君男; 高全良; 甯孝真; 傅學琳; 李慶雲; | 書刊名 | 中華民國小兒科醫學會雜誌 |
卷 期 | 38:6 民86.11-12 |
頁 次 | 頁454-462+499 |
分類號 | 414.84 |
關鍵詞 | 輪狀病毒; VP7血清型; 酶連免疫試驗; 反轉錄-聚合酶連鎖反應; Rotavirus; VP7 serotype; ELISA; RT-PCR; |
語 文 | 英文(English) |
英文摘要 | Serotype specificity of human rotaviruses in Taiwan remained unclear. This study has established three methods for identification of G serotypes, fluorescent focus neutralization test (FFN), enzyme-linked immunosorbent assay by incorporating VP7 (G) serotype-specific monoclonal antibodies (ELISA-MAb) and reverse transcription-polymerase chain reaction (RT-PCR). FFN is a reference standard test or determination of G serotype. RT-PCR and ELISA-MAb were used for serotyping of rotavirus isolates and appeared to be accurate and more sensitive than FFN. Nevertheless, for serotyping. the rotaviruses present in clinical stool samples, ELISA-MAb was not satisfactory, only 65.5% of the 264 specimens could be serotyped. The sensitivity of serotype prediction by RT-PCR was 89.4%. When both ELISA-MAb and RT-PCR were taken into account, G serotype could be predicted for 93.2% of the specimens. Serotyping results were comparable with the RNA profiles. Rotaviruses with similar RNA electrophoretic pattern had the same serotype specificity. RNA patterns could be used as a reference for prediction of serotypes. Because ELISA is easy to perform and less expensive, it would be suggested as a screening test RT-PCR could be used for samples which could not be serotyped by ELISA-MAb. Furthermore, RT-PCR is especially valuable when serotype-specific monoclonal antibodies are not available. |
本系統中英文摘要資訊取自各篇刊載內容。