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題名 | Expression of Epidermal Growth Factor Receptor in Human Middle Ear Cholesteatoma=中耳膽脂瘤之上皮細胞生長因子接受器的表現 |
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作者姓名(中文) | 許英哲; 何坤瑤; 蔡志仁; 李家和; 王凌峰; 吳淑釧; 郭文烈; 蔡世盟; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 19:10 2003.10[民92.10] |
頁次 | 頁497-502 |
分類號 | 416.821 |
關鍵詞 | 上皮細胞生長因子接受器; 中耳膽脂瘤; Epidermal growth factor receptor; Middle ear cholesteatoma; |
語文 | 英文(English) |
中文摘要 | 中耳膽脂瘤在臨床上對於聽小骨和顳骨具有相當的侵蝕性,而治療上通常必須以手術清除中耳腔所有的上皮成份。上皮細胞生長因子接受器(epidermal growth factor receptor,EGFR)是一種已知分子量 170-180 kd 的醣蛋白,而且其密度與角質細胞的分化能力及分化狀態相關。本研究利用免疫組織化學酵素抗體法(avidin-biotin-peroxidase complex,ABC method)以 EGFR 單株抗體免疫染色,評估 29 例膽脂瘤與 34 例耳廓後皮膚兩者組織上皮內 EGFR 陽性細胞之分佈表現。在中耳膽脂瘤上皮組織中,EGFR 呈陽性百分比在 basal layer、parabasal layer 及 upper layer 分別為 79%(23 例)、66%(19 例)及 62%(18 例),而在耳廓後皮膚上皮組織中,其陽性率分別為85%(29例)、79%(27 例)及 79%(27 例)。利用卡方檢定 EGFR 陽性細胞於各層之表現比率,兩組間並無統計學上的差異。然而我們觀察到膽脂瘤上皮組織中 EGFR 的免疫陽性反應自表皮基底層往上層有一強度梯度性(intensity gradient)的存在。本研究結果顯示,上皮細胞生長因子接受器在膽脂瘤中並無異常排列。 |
英文摘要 | Middle ear cholesteatoma is destructive to auditory ossicles and temporal bone, and treatment usually includes surgical removal of all epithelial content in the tympanomastoid cavity. Epidermal growth factor receptor (EGFR) is a 170 kd to 180 kd transmembrane glycoprotein and its distribution density is related to the ability of the keratinocytes to differentiate and their state of differentiation. We used the avidin-biotin complex technique and EGFR monoclonal antibody to evaluate the expression of EGFR in 29 cases of cholesteatoma and 34 samples of normal postauricular skin. Of patients with cholesteatoma, 79% (23 cases) had EGFR-positive cells in the basal layer, 66% (19 cases) in the parabasal layer, and 62% (18 cases) in the upper layer of the epithelial tissue. Among patients with normal postauricular skin, 85% (29 cases) had EGFR-positive cells in the basal layer, 79% (27 cases) in the parabasal layer, and 79% (27 cases) in the upper layer of the epithelial tissue. No statistical difference in EGFR expression between each layer of cholesteatoma and postauricular skin was noted. However, there was an intensity gradient of positive EGFR immunoreactivity from the basal to the higher layers in cholesteatoma. Our results showed that the distribution of EGFR in middle ear cholesteatoma is not deranged, but is similar to that in normal skin tissue. |
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