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| 題 名 | 藍斑核調節孤獨核感壓接受性神經細胞活性之探討=Inhibitory Modulation of Locus Coeruleus on Baroreceptive Neurons in the Nucleus Tractus Solitarii |
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| 作 者 | 施承典; 華瑜; 陳慶鏗; 廖美齡; | 書刊名 | 大仁學報 |
| 卷 期 | 24 2003.12[民92.12] |
| 頁 次 | 頁1-17 |
| 分類號 | 398.2 |
| 關鍵詞 | 藍斑核; 孤獨核; 感壓反射; 神經元放電活性; Locus coeruleus; Nucleus tractus solitarii; Baroreceptor reflex; Extracellular singe-neuron recording; Baseline spike activity; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 藍斑核(locus coeruleus)在中樞心血管系統之神經性控制上扮演一個重要的調節角色。利用生理及藥理學方法,本實驗室過去發現藍斑核對中樞調控心血管系統中一個重要而基本的管制機制-感壓反射(baroreceptor reflex),具有抑制作用。再者,孤獨核(nucleus tractus solitarii)為感壓接受器傳入神經纖維在中樞神經系統形成第一個聯會(synapse)的所在。因此,以藍斑核抑制感壓反射之作用為基礎,本論文主要目的是探討是否藍斑核可藉由降低孤獨核感壓接受性神經細胞活性,造成感壓反射反應之抑制作用。所用動物為成熟雄性Sprague-Dawley大白鼠,先以腹腔注射戊巴比妥鈉鹽(pentobarbital sodium, 40 mg/kg, i.p.)麻醉,並再以連續靜脈滴注方式補充戊巴比妥鈉鹽(20 mg/kg/h, i.v. infusion),以維持動物在適度麻醉狀態,且配合神經藥理、電生理學等評估方法進行研究。 利用靜脈注射phenylephrine(5.0µg/kg)引發之短暫高血壓,可明顯影響大部分(118/123)尾端孤獨核神經細胞之自發性放電活性。此等現象大致分成兩類:一是增加細胞之興奮性(type I, n=81);另一則是抑制其神經元放電活性(type II, n=37)。進一步電刺激藍斑核(10-20µA和10-20 Hz, 1ms, 10-s train),在不影響孤獨核感壓接受性之基礎神經細胞活性下,大部分type I和type II神經細胞對血壓變化之反應性則有明顯抑制現象;同時評估其感壓反射反應之作用,發現在此條件下電刺激藍斑核對感壓反射反應具有抑制作用。這些實驗結果顯示大多數藍斑核可藉由降低孤獨核感壓接受性神經細胞對動脈血壓變化之反應性,造成感壓反射反應之抑制作用。 |
| 英文摘要 | The present study delineated the cellular mechanism at nucleus tractus solitarii (NTS) that may underlie the suppression of baroreceptor reflex (BRR) by locus coeruleus (LC) using extracellular singe-neuron recording in anesthetized male Sprague-Dawley rats. A significant number (118/123) of caudal NTS neurons responded to activation of the baroreceptors by transient hypertension induced by intravenous injection of phenylephrine (5.0 µg/kg) with either an increase (type I, n=81) or decrease (type II, n=37) in their basal spontaneous discharge. Whereas electrical stimulation of LC (10-20 µA and 10-20 Hz, 1ms, 10-s train) did not significantly alter the baseline spike activity of NTS neurons, activation of the LC significantly suppressed the responsiveness of arterial pressure-related NTS neurons to baroreceptor activation (n=73), accompanied by an appreciable inhibition of the sensitivity of BRR response. These results indicate that the LC may suppress the BRR response by an inhibitory modulation of the responsiveness of arterial pressure-related neurons in the NTS to baroreceptor activation. |
本系統中英文摘要資訊取自各篇刊載內容。