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題名 | 大腸桿菌「質體載體」製作釋疑=A Practical Guide for Making E.coli Plasmid Cloning Vectors |
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作者 | 李炎; | 書刊名 | 新竹師院學報 |
卷期 | 17 民92.12 |
頁次 | 頁101-118 |
分類號 | 368 |
關鍵詞 | 聚合連鎖反應; 選殖媒體; 質體載體; 遺傳工程; Genetic engineering; PCR; Plasmid cloning vector; Cloning vector construction; |
語文 | 中文(Chinese) |
中文摘要 | 在實驗室執行大腸桿菌(Escherichia coli-E. coli)質體(plasmid)載體(亦稱選殖媒體,或嫁接媒體-vector)研發時,學生常會有3項困擾。第1為:標的基因排序由何處來?第2為:標的基因序列前後黏接尾(sticky ends)如何形成?第3為:載體須具何種特性方為完整?由何處取得?本文即在逐一說明上述問題。 |
英文摘要 | Students usually have three questions when they are making E. coli plasmid cloning vectors in the lab. How can they get the specific target gene sequence? How the sticky ends formed onto the target sequence? What are the characteristics for a complete plasmid vector? And, where can they get a primary cloning vehicle? This article listed stepwise methodology to solve these problems. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。