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題名 | Novel PCR Primers for Specific Detection of C1, C2 and C3 Enterotoxin Genes in Staphylococcus Aureus=新設計聚合酶連鎖反應引子對金黃色葡萄球菌腸毒素C1、C2及C3型基因之檢測 |
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作者 | 蕭明晃; 陳桐榮; 曾浩洋; Hsiao, Ming-haung; Chen, Tong-rong; Tsen, Hau-yang; |
期刊 | 藥物食品分析 |
出版日期 | 20031200 |
卷期 | 11:4 2003.12[民92.12] |
頁次 | 頁338-343+356 |
分類號 | 412.37 |
語文 | eng |
關鍵詞 | 聚合酶連鎖反應引子; 金黃色葡萄球菌; 腸毒素C1、C2及C3; Staphylococcus aureus; SEC1; SEC2; SEC3; PCR primers; |
中文摘要 | 利用掃描式電子顯微鏡及能譜儀CSEM-EDS) 可快速篩選二氧化硫殘留量過高之市售乾燥金針。實驗結果發現超過3%亞硫酸納處理,或亞硫酸銅浸潰後未經漂水之金針樣品,經乾燥後在掃描式電子顯徵鏡下,金針表面可觀察到顆粒狀沈澱物,以X-ray元素分析證實沈澱物為亞硫酸鈉。將無沈澱物之金針樣品其SEM-EDS所測得之硫/碳值,與改良式Monier-Williams法測得之二氧化硫量作線性回歸,發現此兩種測定方法有良好之相關性 (R2=0.8994) 。 |
英文摘要 | Staphylococcus aureus is the major pathogen that causes clinical infection as well as food poisoning. Enterotoxin produced by Staphylococcus aureus strains are, among others, staphylococcal enterotoxin (SE) A, SEB, SEC, SED, SEE, SEG, SEH, SEI, SEJ, SEK, SEL and SEM. For SEC, there are three major antigentically distinct SEC subtypes, i.e. SEC1, SEC2 and SEC3, in addition to other molecular variants. The nucleotide sequence homology between SEC1, SEC2, and SEC3 genes is higher than 97%; however, we were able to develop a second set of PCR primers that allowed us to differentiate the three SECs, i.e., SEC1, SEC2 and SEC3, S. aureus strains. These PCR primers and their combinations were C12F/C1BR, C12F/C23R and C3BF/C23R, for specific detection of the SEC1, SEC2 and SEC3 genes of S. aureus strains, respectively. Using these primers, we examined 39 SEC S. aureus isolates, which were obtained from foods that were likely the source of food-borne outbreaks between 1995 and 1997 in central Taiwan, and from the patients of the outbreaks. The results were consistent with those obtained from the first set of primers reported earlier, indicating that the present primers could be used for specific detection of these three toxin types. |
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