頁籤選單縮合
題 名 | Optimized Conditions for Growth and Fermentation of Helicobacter Pylori=幽門螺旋桿菌培養與發酵之最佳條件 |
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作 者 | 王錦堂; 張稚笙; 楊智欽; 林肇堂; 王德宏; | 書刊名 | 微免與感染雜誌 |
卷 期 | 31:3 1998.09[民87.09] |
頁 次 | 頁146-150 |
分類號 | 414.83 |
關鍵詞 | 幽門螺旋桿菌; 培養; 發酵; Helicobacter pylori; Culture; Fermentation; |
語 文 | 英文(English) |
中文摘要 | 為得知幽門螺旋桿菌培養之最佳條件,本研究比較幽門螺旋桿菌在厭氧培養箱及 厭氧培養缸之生長情形及分離率,結果胃組織切片之分離率在厭氧培養箱中高於厭氧培養缸 ( 28 ╱ 30 比 21 ╱ 30 ),且菌株在厭氧培養箱之生長速率也高於厭氧培養缸。比較培 養基中各種不同血清與非血清添加劑的生長情形顯示大多數菌株在添加 5 %的小牛血清的 培養基中生長最好,而添加 0.2 %的β -cyclodextrin 則次之。發酵槽大量培養的結果顯 示常用之消泡劑 polypropylenglycol 明顯抑制幽門螺旋桿菌的生長, 以 5 % O �砥B 10 % CO �砟� 85 % N �租V合氣連續充氣為得到大量細菌與蛋白質之最佳發酵條件。 |
英文摘要 | This study attempted to optimize the growth conditions of Helicobacter pylori by comparing the growth of H. pylori in an anaerobic jar and an incubator. It was found that the primary isolation rate of H. pylori from gastric biopsies was higher in an anaerobic incubator (28/30) than in an anaerobic jar (21/30). In addition, growth rate in the subsequent passage was also higher in an incubator. By comparing different supplements in the liquid media, it was found that most strains grew best in Brucella broth containing 5% fetal calf serum, and some strains grew well with the supplement of 0.2% of β -cyclodextrin. A large scale culture using a bioreactor indicated that a defoaming agent, polypropylenglycol, profoundly inhibited the growth of H. pylori. However, fermentation could be successfully performed by continuously infusing mixed airs composed of 5% O ��, 10% CO �� and 85% N ��. Moreover, the production of secreted proteins of H. pylori strains grown in the bioreactor was the same as that in an anaerobic jar. Results presented herein can be used to further optimize conditions for enhancing the growth of H. pylori. |
本系統中英文摘要資訊取自各篇刊載內容。