查詢結果分析
來源資料
相關文獻
- Effects of Infrared and Low-Power Laser Irradiation on Cell Viability, Glutathione and Glutathione-Related Enzyme Activities in Primary Rat Hepatocytes
- Laser-Assisted Hatching of Embryos in Women of Advanced Age after in Vitro Fertilization: A Preliminary Report
- 光電指揮系統技術
- 雷射耳穴治療脊髓損傷患者疼痛的立即效果
- Fabrication processes for an AlGaAs/InGaP/AlGaAs Visible LD Grown by Liquid phase Epitaxy
- 紅外線雷射在生物科技上應用
- 半導體雷射:光電元件的特性及使用方法
- 半導體雷射:光電元件的特性及使用方法
- 光電技術應用新趨勢
- 生醫應用Er:YAG晶體模組化雷射系統
頁籤選單縮合
題 名 | Effects of Infrared and Low-Power Laser Irradiation on Cell Viability, Glutathione and Glutathione-Related Enzyme Activities in Primary Rat Hepatocytes=紅外線及低能量雷射照射對大白鼠初代肝細胞之生存力及麩胱甘冴相關代謝酵素活性之影響 |
---|---|
作 者 | 高木榮; 沈立言; | 書刊名 | 臺灣醫學會雜誌 |
卷 期 | 102:7 2003.07[民92.07] |
頁 次 | 頁486-491 |
分類號 | 416.246 |
關鍵詞 | 肝細胞; 紅外線; 雷射; Cell survival; Glutathione; Hepatocytes; Infrared rays; Lasers; |
語 文 | 英文(English) |
英文摘要 | Background and Purpose: Both infrared and low-power laser have been applied to improve circulation, wound repair, and pain control. Infrared and low-power laser therapies have the potential for stimulating enzyme activities which might contribute to increased glutathione (GSH) concentration and provide protection against oxidative damage. This study investigated cell viability, and GSH and its related enzyme activities in rat hepatocytes after irradiation. Methods: Hepatocytes were isolated from 8-week-old male Sprague-Dawley rats and the cultures were divided into infrared, laser, and control groups. The cells were treated with infrared and low-power laser at a distance of 35 cm for 20 minutes. The cell morphology, lactate dehydrogenase (LDH) leakage, lipid peroxidation, GSH concentration, GSH peroxidase, GSH reductase (GRd), and GSH S-transferase activities were measured after irradiation. Results: The morphology and LDH leakage of hepatocytes in the irradiation groups did not differ significantly from those of the control group. After infrared irradiation, a significant decrease in thiobarbituric acid-reactive substances and an increase in GSH concentration were found after 48 hours of incubation compared to the control group (p<0.05). Furthermore, laser irradiation resulted in a significant increase in GRd activity after 48 hours of incubation compared to the control group (p<0.05). A 48-hour incubation period produced greater GRd activity in all groups compared to a 24-hour period (p<0.05). Conclusions: Irradiation did not damage rat hepatocytes in this study. Infrared was shown to stimulate GSH production, while laser irradiation increased GRd activity. |
本系統中英文摘要資訊取自各篇刊載內容。