查詢結果分析
來源資料
相關文獻
- Detection and Treatment of Mycoplasma Contamination in Cultured Cells
- 凍解及新鮮豬黃體細胞對激黃體物質於體外分泌孕酮之效應
- 影響斜夜蛾細胞株生產桿狀病毒之因子
- 進行性肌肉萎縮症中藥療效之評估--肌胚細胞培養及動物實驗之研究(第一年)
- 生技藥品工廠建廠設計
- 豬眼虹膜色素上皮細胞之分離與培養
- 細胞培養中黴漿菌污染之檢測與去除
- Cycling Growth Characters in Embryogenic Cell Suspension Culture of Banana AAA Cavendish Subgroup and AAB Cultivars
- Culture of Human Primary Airway Epithelial Cells
- 柴胡皂苷對FSC激活及合成細胞外基質的實驗研究
頁籤選單縮合
題名 | Detection and Treatment of Mycoplasma Contamination in Cultured Cells=細胞培養中黴漿菌污染的檢測及清除 |
---|---|
作者姓名(中文) | 容萱; 王師怡; 楊伊雯; 薛丁瑋; 楊薇儒; 王予豪; 王馨世; | 書刊名 | 長庚醫學 |
卷期 | 26:4 2003.04[民92.04] |
頁次 | 頁250-258 |
分類號 | 415.121 |
關鍵詞 | 細胞培養; 黴漿菌污染; Cell culture; Mycoplasmal contamination; |
語文 | 英文(English) |
中文摘要 | 背景:黴漿菌(Mycoplasma)常污染細胞培養,是宿在哺乳類細胞之內小體(endosomes)中最小的原核生物。不同的研究報告指出,大約有15到80%的細胞培養遭受此菌污染在本文中,我們報告在我們實驗室中篩檢與消除污染細胞之黴漿的成功經驗。 方法:巢複式聚合?鏈結反應(nested PCR)與三種顯微檢視-相位差、螢光、前別阻礙位差(DIC),一起用來偵測可能的黴漿菌污染。 結果:在15株細胞中,我們鑑定出6株細胞受到污染。以兩種抗生素-屬macrolide的tiamulin與屬四環素的minocycline治療三週,可有效地去除黴漿菌的污染。這些結果都經過巢複式聚合?鏈結反應與螢光顯微鏡的雙重確認。 結論:從培養細胞中萃取基因體DNA,再用巢複式聚合?鏈結反應偵測,是一種快速及靈敏的黴漿菌檢測法。而以上述兩種抗生素的複合治療能徹底消除污染培養細胞的黴漿菌。因為容易操作,聚合?鏈結反應及/或DAPI營光染色適合經常使用培養細胞模式的研究室,用來檢測黴漿菌之污染。 |
英文摘要 | Background: Mycoplasmas, the smallest and simplest prokaryotes that reside in endosomes of mammalian cells, are widespread contaminants found in cell cultures. About 30% of all cell cultures, varying from 15 to 80%, are reportedly contaminated with muycoplasmas. Here, we present our experience in successfully detecting and treating mycoplasmal infection in various cell lines. Methods: The nested polymerase chain reaction (PCR) detection and microscopic examination, including phase-contrast, fluorescent, as well as differential interference contrast, were used for detecting potential mycoplasma contamination of cell lines used in our laboratory. As soon as mycoplasma was identified, antibiotic treatment was initiated. Results: Mycoplasmal contamination was detected in six of 15 cell lines using the nested PCR amplification of mycoplasma DNA, which was further demonstrated using 4, 6-Diamidino-2-phenylindole (DAPI) staining and fluorescent microscopy. Alternate treatment with two antibiotics, macrolide (tiamulin) and tetracycline (minocycline), effectively eliminated mycoplasma, which was validated by both PCR and microscopic studies. Conclusions: The nested PCR using genomic DNA extracted from culture cells as templates is a rapid and sensitive method for detecting mycoplasma contamination. Treatment with combined antibiotics can completely readicate mycoplasmal infection from cultured cells. For the ease of use, PCR and/or DAPI staining appear suitable for detecting potential mycoplasmal contamination in laboratories that rely heavily on the cell culture system. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。