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題名 | Morphology and Growth Kinetics of Human Pterygium Fibroblast in Primary Culture=人類眼翳纖維母細胞之初級培養的細胞形態與生長動力學研究 |
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作者姓名(中文) | 戴明正; 陳喬鴻; 葉豐順; 張正忠; | 書刊名 | Journal of Medical Sciences |
卷期 | 23:1 2003.02[民92.02] |
頁次 | 頁23-27 |
分類號 | 416.74 |
關鍵詞 | 眼翳; 纖維母細胞; 初級培養; Fibroblast; Primary culture; Pterygium; |
語文 | 英文(English) |
英文摘要 | Background: To establish primary culture and investigate the characteristics of human pterygium fibroblast in vitro. Methods: The morphology of fibroblast was studied with Giemsa stain, scanning electron microscopic (EM), transmission EM and immunostaining of cytoskeleton. Growth kinetic studies were evaluated by growth curve, labeling index, cell cycle, doubling time, saturation density, plating efficiency and anchorage independent test. Results: We successfully establish a primary culture of pterygium fibroblasts in vitro. The fibroblast revealed elastodysplasia and dystrophy. The doubling time was 20 h and saturation density was 8 x 10² cell per cm² . The plating efficiency was 36-43%, which was higher than that of normal cells (28-34%). Conclusions: Abnormal morphology and growth behavior of pterygium fibroblasts may explain the progressive nature of clinical pattern. This model could be used for further studies on pathogenesis, prevention and treatment of pterygium. |
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