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| 題 名 | 瘤胃真菌Neocallimastix frontalis W-1分離培養與纖維分解能力之研究=The Isolation and Fiberolytic Ability of Rumen Fungus Neocallimastix frontalis W-1 |
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| 作 者 | 陳又嘉; 黃志?; 許瑞祥; | 書刊名 | 中華生質能源學會會誌 |
| 卷 期 | 21:3/4 民91.12 |
| 頁 次 | 頁119-125 |
| 分類號 | 379.1 |
| 關鍵詞 | 瘤胃真菌; 碳源耐受度; 纖維分解能力; Carbon source tolerance; Fiberolytic ability; Neocallimastix frontalis; Rumen fungus; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 瘤胃真菌被發現生存於反芻動物的消化道中,他們可產生多種不同纖維分解酵素與木聚醣分解酵素幫助宿主分解植物細胞壁多醣作為能量來源,因此被認為可成為高活性纖維分解酵素與木聚醣分解酵素的來源。本研究,自行政院農業委員會花蓮種畜繁殖場所飼養的水牛瘤胃液分離出W-1菌株,在動孢子時期具有多鞭毛動孢子,在菌絲體世代具有單中心體菌體與絲狀假根等形態特徵,與已發表的Neocallimastix frontalis Braune所記載的形態特徵相符,因此將W-1菌株歸類成此種。在碳源耐受度測試方面,當以濾紙、羧甲基纖維、葡聚醣、纖維雙糖、木聚醣等作為碳源時,W-1菌株生長狀況與以葡萄糖作為碳源時相近。此外,W-1菌株亦可生長於以果膠、結晶性纖維、木質素作為唯一碳源的培養基,顯示N. frontalis W-1菌株可利用多種不同形式的植物細胞壁多醣。在纖維分解酵素與木聚醣分解酵素方面,當以稻桿作為誘導基質時,呈現較佳的誘導狀況;Exoglucanase、endoglucanase、beta-glucosidase與木聚醣分解活性分別為5.7 U/ml、101.7 U/ml、5.3 U/ml與2015 U/ml。 |
| 英文摘要 | Anaerobic rumen fungi are found in the digestion tract of herbivors. They process divers fiberolytic enzymes (e.g. cellulase and xylanase) to help hosts digest plant cell-wall polysaccharides. Thus, these fungi are regarded as one of the best potential sources for providing cellulase and xylanase. In this study, we had isolated an anaerobic fungus, W-1 from the rumen of Taiwan water buffalo. This strain developed polyflagellate zoospores, monocentric thalli, and filamentous rhizoid system, these morphological characteristics resemble to thsoe of Neocallimastix frontalis Braune. W-1 strain grew in basal medium with various carbon sources such as filter paper, carboxylmethyl cellulose, beta-glucan (barely) cellulobiose, xylan (Birchwood and Oat), and rice straw source as that wtih glucose as sole carbon source. Besides, this strain could utilize Avicel, pectin, and lignin as sole carbon source, When N. frontalis W-1 was cultivated with different substrates for enzyme induction, rice straw induction showed the highest activities and each ml of broth contained 5.7 U, 101.7 U, 5.3 U and 2015 U in exoglucanase, endoglucanase, beta-glucosidase and xylanase, respectively. |
本系統中英文摘要資訊取自各篇刊載內容。