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題名 | Modification of Methyl Methacrylate Triblock Copolymer with Chelating Agent and Its Application in Protein Affinity Coprecipitation=甲基丙烯酸三團聯共聚物螯合改質及其於蛋白質親和性共沈澱之應用 |
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作 者 | 黃祥鳴; 陳文逸; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 33:4 2002.07[民91.07] |
頁次 | 頁397-405 |
分類號 | 460.02 |
關鍵詞 | 三團聯共聚物; 螯合; 蛋白質; 親和性共沈澱; 甲基丙烯酸三團聯; Methyl methacrylate triblock copolymer; Iminodiacetic acid; Affinity coprecipitation; Protein purification; |
語文 | 英文(English) |
中文摘要 | 近年來藉由官能基轉移聚合法,多功性的甲基丙烯酸三團聯高分子已可以被精確的合成各種特定的團聯順序和大小。由於此共聚物上的基團可具有不同的分子量、親疏水以及靜電特性,因此可以藉由分子工程的設計來展現多樣化的溶液特性,並且加以應用於生化分離的程序上。本研究選定B8M12A16高分子加以改質,使其接上螫合觸手乙胺二醋,並且以加鍵結銅離子後用以分離蛋白質,而改質的結果可藉由物理和化學特性的改變來驗證。初步的蛋白質共沈澱實驗計對Lysozyme、Myoglobin和Hemologbin來進行測試,結果顯示B8M12A16-IDA-Cu可以和Hemoglobin親和性共沈澱,但對於Lysozyme和Myoglobin則沒有明顯的專一親和性,這是由於親和性共沈澱的產生和個別蛋白質表面暴露的胺基酸數目,以及高分子改質的程度有關。本研究證實了利用改質的甲基丙烯酸高分子親和性沈澱的可行性。 |
英文摘要 | Methyl methacrylate triblock copolymer, which can be synthesized by group transfer polymerization, is a multifunctional polymer. Triblock copolymer consists of blocks of monomer with various properties, such as molecular weight, hydropobicity and charge. It can be utilized for various bioseparation processes. Furthermore, the arrangements of sequences and sizes of the blocks provide rich solution behavior for various purposes. The concepts of molecular engineering and molecular design can be applied to the synthesis of this triblock copolymer and its application. In this research, copoloymer B8M12A16 was modified using iminodiacetic acid to chelated copper ions for protein affinity coprecipitation, where B represents methyl methacrylate group, M is 2-(dimethylamino) ethyl methacrylate group and A is methacrylic acid group. The chemical structure and the physical properties of the modified copoloymer were investigated to ensure that the copolymer was modified accordingly. Finally and most importantly, preliminary coprecipatation experiments that involve three different kinds of proteins-lysozyme, myoglobin and hemoglobin-are preformed. Results show that Hemoglobin can be coprecipitated with B8M12A16-IDA-Cu by affinity interaction but not for Lysozyme or Myoglobin. These results are attributed to the various numbers of exposed histidine residues of protein and the degrees of modification of the chelating agent in the triblock copolymer. In conclusion, the potential for utilizing modified triblock copolymer for slective affinity coprecipitation was revealed. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。