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頁籤選單縮合
題名 | Determination of Territtrem B in Rice Media with Different Strains of Aspergillus terreus by Chromatography=利用層析法探討不同Aspergillus terreus菌株於米中產生土震素B之能力 |
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作者姓名(中文) | 方信裕; 危貴金; 彭福佐; | 書刊名 | 藥物食品分析 |
卷期 | 10:3 2002.09[民91.09] |
頁次 | 頁159-163 |
分類號 | 412.37 |
關鍵詞 | 土震素; 土麴菌; 薄膜層析法; 高效能液相層析法; Territrem; Aspergillus terreus; TLC; HPLC; |
語文 | 英文(English) |
中文摘要 | 土震素B(territrem B)為土麴菌(Aspergillus terreus)產生之震顫性微菌毒素,可導致動物全身性顫抖、流涎、抽搐、甚至導致死亡,其作用機制為抑制乙醯膽鹼酵素(acetylcholinesterase)的活性。本研究目的在於探討食品工業發展研究所菌種保存中心之17株A. terreus於米基中產生土震素B的能力。首先從薄膜層析法及高效能液相層析法進行分析,發現僅編號32111與32664之菌株可產生與土震素B類似之藍色螢光物質,進一步將此藍色螢光物質以質譜儀鑑定為土震素B。土震素B的含量以高效能液相層析法配合C18逆向層析管柱及於335nm波長下測定。土震素B之標準線性公式為Y=1428.5X+306.0(R2=0.9998),其中Y為土震素B於高效能液相層析儀之面積,X為土震素B的濃度,顯示良好的線性關係;同日問及異日開之相對標準偏差分別為1.6~2.8%及0.6~2.39%,顯示具有良好之準確性及精確性;添加回收率在91.5~95.2%之間,顯示氯仿可作為米基萃取液;利用此方法可知A. terreus 32111及32664產生土震素B含量分別為0.2及0.3 mg/100g米。從地源性而言,產生土震素B之A. terreus 32111及32664兩株菌為台灣本土之菌株,其它的株分別來自不同國家之A. terreus,無法產生土震素B。 |
英文摘要 | Territrem B is a tremogenic mycotoxin produced by Aspergillus terreus. Animal studies have demonstrated that territrem B caused whole body tremor, salivation, convulsion and death. Territrem B is also a potent irreversible inhibitor of acetylcholinesterase (AchE, E. C. 3.1.1.7). The aim of the present study is to assess the ability of strains of Aspergillus terreuss to produce territrem B in the rice media, which were obtained from Food Industry Research and Development Institute in Taiwan. In the 17 strains of A. terreus, only No. 32111 and 32664 strains produced territrem B-like blue fluorescence compound by TLC and HPLC analyses. Later on, this compound was identified as territrem B by mass spectroscopy. The amount of territrem B was determined by reverse phase HPLC on a C18 column with UV detection at 335 nm. The linear regression coefficient of the standard curve (Y=1428.5X+306.0) for territrem B at concentration ranging from 0 to 250 ng/L is 0.9998. In rice media, the recovery rates for territrem B at 50, 100 and 200 ng/L ranged from 91.5% to 95.2% with a coefficient of variation less than 2.1%. The relative standard of territrem B ranged between 1.6-2.8% (intra-day) and 0.6-2.3% (interday). In rice culture of A. terreus 32111 and 32664 strains, the contents of territrem B were 0.20 and 0.31 mg/100 g rice, respectively. From the above results, it can be concluded that this HPLC method for determination of territrem B in rice media is suitable and only domestic strains can produce territrem B. |
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