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題名 | Determination of Hesperetin and Its Conjugate Metabolites in Serum and Urine=血清與尿液中橙皮素及其結合態代謝物之定量 |
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作者 | 楊啟裕; 蔡尚元; 李珮端; 顏秀芳; 錢組明; 徐素蘭; Yang, Chi-yu; Tsai, Shang-yuan; Lee Chao, Pei-dawn; Yen, Hsiu-fang; Chien, Tsu-ming; Hsiu, Su-lan; |
期刊 | 藥物食品分析 |
出版日期 | 20020900 |
卷期 | 10:3 2002.09[民91.09] |
頁次 | 頁143-148 |
分類號 | 414.51 |
語文 | eng |
關鍵詞 | 橙皮苷; 橙皮素; 結合態代謝物; 高效液相層析法; 酶解; Hesperidin; Hesperetin; Serum; Urine; HPLC; |
中文摘要 | 橙皮苷與橙皮素為雙氫黃酮類,具抗胃潰瘍、抗氧化及抗癌等作用,存在於許多柑橘屬果實中。橙皮素為橙皮苷之苷元代謝物。本研究開發高效液相層析法檢測口服橙皮素、橙皮苷與陳皮水煎劑後之血清或尿液於酶水解前後之橙皮素含量,以分別定量其中之橙皮素及其結合態代謝物。酶解使用β-葡萄糖醛酸酶/硫酸酶之混合時於37°C下進行,採避光並添加抗壞血酸。高效液相層析法採用LiChrospher 100 RP-18e column,以5,7-dimethoxycoumarin為內標準,血清分析之移動相組成為乙腈/1%醋酸水溶液 (36/65, v/v),尿液分析之移動相組成為甲醇/乙腈/1%醋酸水溶液 (17/26/57, v/v)。此等分析方法快速、靈敏、再現性、精密度、準確度俱佳且回收率高。因此,本方法可提供橙皮苷、橙皮素或含彼等成分之柑橘屬食品或中草藥之藥物動力學研究。 |
英文摘要 | Hesperidin and hesperetin are flavanones in many Citrus fruits with antiulcer, antioxidation and anticancer activities. Hesperetin is the aglycone and a metabolite of hesperidin. HPLC analysis was developed for the determination of hesperetin and its conjugate metabolites in serum and urine prior to and after hydrolysis following oral administrations of hesperetin, hesperidin, or decoction of Pericarpium Citri Reticulatae (Chenpi). The hydrolysis was conducted away from light by using glucuronidase/sulfatase in the presence of ascorbic acid at 37°C. The HPLC methods employed a LiChrospher 100 RP-18e column, using acetonitrile/1% acetic acid (36/65) and methanol/acetonitrile/1% acetic acid (17/26/57) as mobile phases for serum and for urine, respectively. For internal standard, 5,7- dimethoxycoumarin was used. The present methods are rapid, sensitive, reproducibility, precise, and accurate with good recoveries. They are applicable for the pharmacokinetic studies of hesperidin and hesperetin as well as Citrus foods or herbs containing them. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。