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題名 | 鉤藤五種萃取物對Kainic acid誘發大鼠腦組織脂質過氧化作用效用之比較=Comparative Effect of Five Extracts of Uncaria Rhynchophylla on Lipid Peroxidation in Kainic Acid--Treated Rat Brain Tissue |
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作者姓名(中文) | 謝慶良; 林慧怡; 李佳容; 唐娜櫻; 江素瑛; 謝慶竇; 林昭庚; |
作者姓名(外文) | Hsieh, Ching-liang; Lin, Hui-yi; Lee, Chia-jung; Tang, Nou-ying; Chiang, Su-yin; Hsieh, Ching-tou; Lin, Jaung-geng; |
書刊名 | 臺灣中醫醫學雜誌 |
卷期 | 1:3 2002.09[民91.09] |
頁次 | 頁11-24 |
分類號 | 414.5 |
語文 | chi |
關鍵詞 | 鉤藤五種萃取物; 脂質過氧化作用; Five extracts of uncaria rhynchophylla; Lipid peroxidation; Kainic acid; |
中文摘要 | 鉤藤是中藥的一種,由於它有平肝熄風的作用,所以傳統中醫常用鉤藤來治療癲 癇。我們先前研究已知鉤藤在大鼠可抑制kainic acid(KA)所誘發的癲癇發作,這個作用 可能與降低大腦皮質的脂質過氧化作用有關,但鉤藤的有效成份至今仍然是一個謎。本研究 的目的是為了進一步瞭解鉤藤的抗癲癇成份,用KA誘發Sprague-Dawley(SD)大鼠大腦皮 質的脂質過氧化,使脂質過氧化物濃度升高,來比較鉤藤五種萃取物的抗脂質過氧化作用之 效用。方法是將6隻SD大鼠腹腔注射pentobarbital(50mg/kg)麻醉後,將它們犧牲取腦, 並剝離出大腦皮質,磨碎製成懸浮液,然後將上清液加入KA(120μg/ml)後,分別加入不 同濃度(1mg/ml,0.1mg/ml,0.01mg/ml)之五種鉤藤萃取物或Vitamin E(10mM), 以 thiobarbituric acid法測定脂質過氧化物的濃度。結果顯示濃度愈高的鉤藤萃取物,其抗 氧化能力愈大。鉤藤萃取物在高濃度(1mg/ml)下,Ur-Ⅰ(鉤藤甲醇抽出物),Ur-Ⅱ( 鉤藤正己烷層萃取物)及Ur-Ⅴ(鉤藤水層萃取物)的抗脂質過氧化作用相似,甚至與 Vitamin E沒有顯著的差別。鉤藤萃取物在濃度0.1mg/ml時,Ur-Ⅲ(鉤藤酸性二氯甲烷層 萃取物)的抗脂質過氧化作用較Ur-Ⅰ或Ur-Ⅴ小。當鉤藤萃取物在0.01mg/ml更小的濃度下 。Ur-Ⅲ和Ur-Ⅳ(鹼性二氯甲烷萃取物)的抗脂質過氧化作用較Ur-Ⅰ小。另外,Ur-Ⅳ 的抗脂質過氧化作用也比Ur-Ⅴ小。無論在1.0mg/ml,0.1mg/ml或0.01mg/ml的濃度下 Ur-Ⅲ和Ur-Ⅳ兩者的抗脂質過氧化作用相似。 我們的結論是,鉤藤抗癲癇之有效成分可能存在於甲醇抽出物、正己烷層萃取物與水層 萃取物中,而非在二氧甲烷萃取物中。 |
英文摘要 | Uncaria rhynchophylla (Ur) is a Chinese herb, has an action of calm the liver and extinguish wind; therefore, it is used to treat epilepsy in Tradition Chinese Medicine. In our previous studies, we found Ur can inhibit kainic acid (KA)-induced epileptic seizures, and this effect of Ur possibly results from inhibition of lipid peroxidation, but the antiepileptic component of Ur remains still unclear. The aim of the present study is to investigate antiepileptic component of Ur, using KA-induced lipid peroxidation of cerebral cortex to increase the levels of lipid peroxide in Sprague-Dawley (SD) rats, and their suppressive effect of lipid peroxidation were compared among five extracts of Ur. A total 6 SD rats were studied, brain of the rats was removed after anesthesia with pentobarbital (50mg/kg i.p.), and the cerebral cortices were separated from the brain. The cerebral cortex was immediately homogenized and centrifuged. Kainic acid (KA, 120μg/ml) was added to supernatants, and then different concentration (1mg/ml, 0.1mg/ml, 0.01mg/ml) of five extracts of Ur and Vitamin E (10mM) was added to the samples, respectively. The lipid peroxide levels were determined by measuring concentration of malondialdehyde. The results indicated that dosage and suppressive effect of lipid peroxidation have a positive correlatively relationship in all five extracts of Ur. In concentration 1mg/ml, similar suppressive effect of lipid peroxidation was observed among Ur-Ⅰ (extract of Ur with methyl alcohol), Ur-Ⅱ (extract of Ur with n-hexane), Ur-Ⅴ (extract of Ur with water) and vitamin E. In concentration 0.1mg/ml, Ur-Ⅰ and Ur-Ⅴ have grater suppressive effect of lipid peroxidation than Ur-Ⅲ (extract of Ur in acid methane dichloride). In concentration 0.01mg/ml, Ur-Ⅰ has greater suppressive effect of lipid peroxidation) than Ur-Ⅲ or Ur-Ⅳ (extract of Ur in alkaline methane dichloride). In addition, Ur-Ⅴ has greater suppressive effect of lipid peroxidation than Ur-Ⅳ. The suppressive effect of lipid peroxidation was similar between Ur-Ⅲ and Ur-Ⅳ in concentration 1.0mg/ml, 0.1mg/ml and 0.01mg/ml. In conclusion, the anticonvulsive component of Ur possibly dissolves in the methyl alcohol, water and n-hexane solution, but not in the methane dichloride solution. |
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