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題名 | Decontamination of Endotoxin on Particulate Implant Materials with Acetic Acid=植體表面受到細菌性內生毒素(脂多醣體LPS)污染的影響 |
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作者 | 李明澍; 陳怡君; 王東美; 郭生興; 王若松; 洪志遠; 林思洸; | 書刊名 | 中華口腔顎面植體雜誌 |
卷期 | 1:1 2001.06[民90.06] |
頁次 | 頁10-20 |
分類號 | 416.96 |
關鍵詞 | 植體顆粒; 脂多醣體; Implant particles; LPS; RAW 264.7; IL-1; TNF-α; |
語文 | 英文(English) |
中文摘要 | 牙科領域廣泛使用生物材料,儘管值體有極佳的成功率,因感染或無菌型鬆脫導致植體失敗仍是臨床上常見的問題。因磨損產生的顆粒致骨質溶解間接造成植體無菌式鬆脫是主要的失敗模式,磨損的顆粒造成肉牙腫性發炎及細胞激素產生、進而刺激噬骨細胞的骨吸收過程。 由細胞壁的衍生物而來的脂多醣體被稱作內生毒素,初認為是影響植體成功率的因素。證據顯示脂多醣體可藉由增加多種細胞激素(如IL-1、IL-6、TNF)來活化巨噬細胞之活性。本實驗的目的在探討內生毒素污染在細胞產物釋出中所可能扮演的角色,此外建立一活體外巨噬細胞模型以研究顆粒造成之細胞反應以及析在分子層級下的訊號路徑和相關細胞激素基因控制之機制。 實驗中,我們使用特別的顆粒、巨噬標的細胞系統,小心控制顆粒特性及細胞反應之變異,並著眼於控制骨頭、結締組織重建過程有關之細胞激素的觀察。基於細胞生存能力及北方點漬法的結果顯示在脂多醣體移除後,對於TNF-α vs IL-1 顆粒會有無法預期的特異細胞激素反應。結論,我們證明內生毒素(LPS)在實驗模型的角色並成功的將LPS污染移除植體顆粒。 |
英文摘要 | Biomaterials are widely used in the practice of medicine and dentistry. The wear debris associated with the biomaterials used for orthopedic and dental implants can cause the body to mount and inflammatory response. In previous studies concerning the pro-inflammatory effects of particulate implant materials, endotoxin - LPS contamination remained an annoying problem because of its ubiquitous presence and potent biological effects. The traditional method for the removal of LPS activity by heating the materials to 180℃ for 4 hr. is not practical because of its detrimental effect to the particle surface. In the study method of boiling in 1% acetic acid for 90 min. was tested. Nine types of particles including different forms of silica, Ti□, Tin, cpTi, Ti-6Al-4V and Co-Cr-Mo were used. Limulus amebocyte lysate (LAL) endotoxin assay showed that even after standard sterilization, all particles were contaminated to some degree with LPS. After boiling in 1% acetic acid all 9 types of particles showed no LPS activity on LAL assay. Cell stimulation assay with the murine peritoneal macrophages cell line. RAW 264.7 confirmed the efficacy of the acid decontamination method, TiN particles without acid treatment showed a pattern of Il-1β and TNF-α stimulation similar to that provoked by LPS alone. After acid treatment, the stimulatory effect of TiN particles was largely abrogated. The cellular responses to silica glass particles were different from those associated with TiN but the effect of acid treatment was still obvious. It is conclude that treatment with 1% acetic acid is an effective method for LPS decontamination of particulate implant materials. |
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