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題 名 | Differential Expression of Cytokine Genes and Apoptosis in Glioma Cell Lines upon Exposure to Bacteria and Lipopolysaccharides=不同細菌及其脂多醣體對腦膠質母細胞瘤中細胞素基因的表現和細胞凋亡的影響 |
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作 者 | 楊倍昌; 楊彩蓮; | 書刊名 | 微免與感染雜誌 |
卷 期 | 31:2 1998.06[民87.06] |
頁 次 | 頁95-100 |
分類號 | 415.9313 |
關鍵詞 | 細菌; 脂多醣體; 腦膠質母細胞瘤; 細胞素基因; 細胞凋亡; Escherichia coli; Streptococcus pyogenes; Lipopolysaccharides; Cytokines; Apoptosis; |
語 文 | 英文(English) |
中文摘要 | 為探討腦膜炎發生過程中腦細胞的變化,我們以大腸桿菌和化膿性鏈球菌及脂多 醣體處理兩株腦膠質母細胞瘤細胞, 然後利用 reverse transcription-polymerase chain reaction 檢測細胞中細胞素基因的表現,並且觀察細胞的凋亡。 結果顯示細胞中 IL-1 β 和 TNF- α基因的表現隨著細胞株、不同細菌處理而有所不同。這兩種細胞素表現的時間動 力學也有差異。然而,這些處理方式對於 IL-6 的表現並無明顯的影響。依細胞數目、細胞 膜翻轉、in situ death detection 等方法觀察證實以大腸桿菌和脂多醣體只能引起很少量 的細胞凋亡,但是化膿性鏈球菌的處理則看不出明顯的影響。另外,fas 基因的表現也不因 細菌處理而有所不同。以上結果顯示:細菌和脂多醣體影響腦細胞的初期以干擾細胞素的表 現為主,而不是導致細胞凋亡。 |
英文摘要 | The influences of Escherichia coli, streptococcus pyogenes and lipopolysaccharides (LPSs) on the expression of cytokines and apoptosis were investigated in two glioma cell lines. IL-1 β and TNF- α genes were modulated by bacteria in a time dependent fashion and their expression levels varied from sources of bacteria and cell lines used. E. coli and LPSs induced apoptosis in a very limited cell population as judged by cell counts, membrane alternation and DNA break in situ. The expression of fas and IL-6 genes was not affected by bacterial components. In conclusion, aberrant expression of cytokines but not apoptosis may be the major responses of the cells of glial origin upon exposure to bacteria and their components. |
本系統中英文摘要資訊取自各篇刊載內容。