查詢結果分析
相關文獻
- 四季蔥健康種苗快速繁殖技術(1)--分蔥潛隱病毒檢測與無病毒新梢之誘導
- Detection of Endogenous Digitalis-Like Immunoreactive Factors in Human Blood
- 即食性沙拉用生菜中Escherichia coli O157:H7之污染
- 核醫臨床免疫分析實驗在內分泌學簡介
- 氣功外氣對人類早期單核細胞株和流行性感冒病毒的效應
- 免疫分析在穀類及穀類食品的黴菌毒素偵測上的應用
- 青葱「桃園三號」之育成
- Effect of Ascorbic Acid on Mouse Plasma Fibronectin Concentration
- 鴨與鵝之卵巢抑制素α-次單元基因表現與血中抑制素含量之探討
- 青蔥栽培管理
頁籤選單縮合
題名 | 四季蔥健康種苗快速繁殖技術(1)--分蔥潛隱病毒檢測與無病毒新梢之誘導=Rapid Multiplication of Virus-Free Vegetatively Propagated Bunching Onion (Allium fistulosum) (1)--Shallot Latent Virus Detection and Virus-Free Shoot Induction |
---|---|
作者姓名(中文) | 楊宏瑛; 鄧汀欽; 張武男; | 書刊名 | 花蓮區農業改良場研究彙報 |
卷期 | 19 2001.11[民90.11] |
頁次 | 頁37-49 |
分類號 | 435.221 |
關鍵詞 | 青蔥; 快速繁殖; 免疫分析; 分蔥潛隱病毒; Bunching onion; Micropropagation; ELISA; SLV; Thidiazuron; TDZ; |
語文 | 中文(Chinese) |
中文摘要 | 利用免疫分析檢測分蔥潛隱病毒(SLV)在蔥苗組織內的均勻分佈,據以發展有效率的ELISA步驟,用來作青蔥健康苗繁殖體系的病毒例行檢測。試以感病之青蔥莖基盤、生長點、生長點帶一枚葉原體、生長點帶二枚葉原體等作為培植體,以培養於固態MS培養基中,只有生長點頂分生組織可獲得無病毒青蔥。對無病毒植株加速增殖方法時,TDZ與NAA同時存於MS培養基中,會減少新梢產生之數目,所以單獨添加TDZ對於誘導新梢產生之效果較佳。莖基盤經TDZ濃度1 mg/L– 5 mg/L處理42日後,約可誘導43-62個新梢,即有最佳誘導新梢數目。TDZ較BA或kinetin有較佳之效果。 |
英文摘要 | Shallot latent virus (SLV) was detected by DTBIA, and evenly distributed in infected bunching onion (Allium fistulosum L.). An ELISA procedure was efficiently assigned for routine virus indexing in healthy seedling mass production system. For culture in MS medium, tissues from SLV infected bunching onion were excised, and the tested explants including stem-disc, apical meristem, meristem with one primordial leaf, and meristem with two primordial leaves. The results showed that only the plantlets derived from apical meristem are SLV virus-free. The rapid multiplication of healthy stock was also established. It was demonstrated that the number of regenerated shoots from stem-disc was reduced when TDZ and NAA combined together in MS media, but TDZ alone would regenerate more shoots. The stem-disc treated with 1-5 mg/L TDZ for 42 days, the optimal number of shoots (43-62) were regenerated. The TDZ had better effect on induction of shoot regeneration than BA or kinetin. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。