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題名 | 老化促進小鼠於不同月齡之老化變化研究=Studies of Aging in Senescence-accelerated Mice at Different Ages |
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作者 | 吳青蓮; 詹吟菁; 陳英茹; 王銘富; | 書刊名 | 中華民國營養學會雜誌 |
卷期 | 26:4 2001.12[民90.12] |
頁次 | 頁248-257 |
分類號 | 361.273 |
關鍵詞 | SAMP8系小鼠; 學習記憶; 海綿樣變性; Senescence-accelerated mice; Learning and memory; Spongy degeneration; |
語文 | 中文(Chinese) |
中文摘要 | 本研究主要探討不同月齡老化促進小鼠(senescence accelerated mice; SAMP8)之老化變化。實驗將SAMP8系小鼠分為3、6、9、12及15月齡等五組,每組雄性與雌性各20隻,餵飼一般固型飼料。實驗期間觀察體重與攝食量,並進行活動量試驗(open field activity test)及單次被動迴避試驗(single-trial passive avoidance test),動物犧牲後,觀察腦部組織病理變化。結果顯示,9月齡組攝食量最高(p < 0.01); 12及15月齡組於實驗後體重有下降之情形。活動量隨測試時間增加而下降。在學習記憶方面,雄性15月齡組經單次被動迴避試驗訓練後,其記憶能力較6月齡組有顯著低下(p < 0.05);而雌性之學習及記憶能力均顯著低於3及6月齡組(p < 0.05)。腦部病理方面,隨著年齡的增長,腦部海綿樣變性呈現愈嚴重的傾向,於12及15月齡組為最高;且其在全腦之變化有由前往後增加的現象,以腦幹與脊髓為主要病變區域。由本實驗結果得知,SAMP8系小鼠伴隨年齡的增長,有學習記憶能力呈逐漸低下及腦部海綿樣變性愈益嚴重之傾向。因此,本實驗針對SAMP8系小鼠建立的基礎資料,可提供日後老化研究之主要參考。 |
英文摘要 | The purpose of this study was to examine variations in learning and memory and brain spongy degeneration with age in senescence-accelerated mice (SAM). Senescence-accelerated mice (P8 strain) were divided into five age groups (containing mice at 3, 6, 9, 12, and 15 months old), and each group included 20 male and 20 female mice. Animals were fed a general solid diet; body weight and food intake were recorded and open field activity test and single-trial passive avoidance were evaluated during the experiment. After the experiment, mice were sacrificed to evaluate the brain histopathology of spongy degeneration. The results show that the 9-month-old group had the highest food intake (p < 0.01). Body weights of the 12- and 15-month-old mice in the single-trial passive avoidance test (p < 0.05) for males. However, in female groups, 15-month-old mice had significantly lower learning ability and memory (p < 0.05). Spongy degeneration of the brain became more serious with age. Vacuoles were mainly observed in the brain stem and spinal cord section, and increased from front to back. To sum up, the results indicate that learning and memory abilities decreased and brain spongy degeneration became more serious with age. Therefore, we conclude that the results of this study may serve a model for future aging studies. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。