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題 名 | 利用DNA分子標識確認落花生種間雜交種(4x×2x)基因組組成與外表型變異間之矛盾性=Identification of the Inconsistence between Genomic Constitution and Phenotypic Variation in Interspecific Hybrids (4x×2x) of Peanut with DNA Marker |
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作 者 | 黃惠娟; 林順福; 謝兆樞; | 書刊名 | 中華農業研究 |
卷 期 | 50:3 2001.09[民90.09] |
頁 次 | 頁12-24 |
分類號 | 434.254 |
關鍵詞 | 落花生; 種間雜交種; 分子標識; Peanut; Arachis hypogaea; Interspecific hybrids; Molecular marker; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究利用RAPD及SSR分子標識來分析落花生不同倍數性種間雜種(4x × 2x)的多型性產生之頻率,並探討不同倍數性之種間親本對後代DNA多型性之貢獻是否有差異存在。試驗結果顯示,使用水稻或玉米的Intra-SSR引子可由PCR增幅落花生基因組約11個DNA片段,而Inter-SSR則可由PCR增幅約7個落花生DNA片段。Intra-SSR及Inter-SSR單一引子產生親本多型性之比率,平均分別為45.4%及45.1%高於RAPD的41.3%。而Intra-SSR及Inter-SSR產生各個條帶之多型性比率分別為34.2%及36.0%,亦高於RAPD的33.6%。而不論是RAPD或SSR的分析,可發現F₁的DNA條帶形式與栽培種母本相近,且其多型性條帶平均有66.8%是來自母本,只有33.2%的條帶是來自野生種父本,此種結果與基因組來源及貢獻量相符,但是卻與種間雜交種之外表型變異傾向於野生種之結果不合。因此,本試驗由分子層次確認支持基因組大小與植株外表型變異之不一致性。建議本試驗結果,可提供作物基因組及基因表現研究與種間雜交利用於品種改良之參考。 |
英文摘要 | In this study RAPD and SSR markers were used to analyze genomic context of interspecific hybrids (A. hypogaea L. × A. spegazzinii L.) of peanut. Frequencies and parental origins of DNA polymorphic bands were investigated for the interspecific hybrids (4x × 2x). The results indicated that about 11 bands were PCR amplified from total genomic DNA of peanuts with intra-SSR primers of rice or corn, and about 7 bands were amplified with inter-SSR primers. The averaged polymorphic rates were 45.4% and 45.1% for a pair of intra-SSR primer and an inter-SSR primer, respectively. However, the averaged polymorphic rate for a RAPD primer was 41.3%. The percentages of a (pair of) primer with polymorphic bands between interspectific parents were 34.2, 36.0% and 33.6% for intra-SSR (rice/corn), inter-SSR, and RAPD primers, separately. About twice (66.8%) polymorphic bands were contributed from allotetraploid parent compared to diploid parent (33.2%) providing molecular evidence of genomic context of interspecific hybrids and inconsistence between genomic contribution and phenotypic variation. This information is valuable for genome and gene expression studies as well as the utilization of interspecific hybridization in plant breeding. |
本系統中英文摘要資訊取自各篇刊載內容。