查詢結果分析
來源資料
相關文獻
- Growth Inhibition and Differential Induction of Human Leukemic U937 Cells by Chinese Medicinal Herbs
- 中藥組織培養之研究:半夏癒合組織的誘導及植物體分化之研究(I)
- 以間葉系幹細胞之肝臟細胞分化技術測試固有成方保肝中藥之療效驗證
- 生長分化因子之生物功能
- Alkali Syenites and Associated Rocks in Chintan, Northern Taiwan: A Reappraisal
- 進行性指掌角化症表皮最終分化及角質層功能異常之探討
- 花生花藥培養的研究(1)--不同物種花生花藥培養癒合組織誘導及芽體分化的探討
- 玉屏風散單味藥及不同組合方劑在體外試驗對單核細胞釋放第2介白質及前列腺素E[feaf]之影響
- 臺灣市售易誤用、混用中藥品之調查
- 中藥對口腔病原菌及牙周病治療效果之評估
頁籤選單縮合
題名 | Growth Inhibition and Differential Induction of Human Leukemic U937 Cells by Chinese Medicinal Herbs=中草藥萃取物對白血病U937細胞之抑制及分化作用 |
---|---|
作者 | 馬嘉軉; 張鴻民; Ma, Chia-yu; Chang, Hung-min; |
期刊 | 藥物食品分析 |
出版日期 | 20041200 |
卷期 | 12:4 2004.12[民93.12] |
頁次 | 頁347-352+373 |
分類號 | 414.51 |
語文 | eng |
關鍵詞 | 中藥; U937細胞; 生長抑制; 分化; 條件培養液; NBT還原力; Growth inhibition; Differentation; U937 cell; Medicinal herb; Condition medium; NBT reducing ability; |
中文摘要 | 本實驗探討熱水及80%乙醇萃取之中草藥對U937細胞之抑制及分化作用。篩選模式為以多種不同濃度 (200-800μg/mL) 的中草藥刺激人類周邊血液單核細胞,再取其單核細胞條件培養液 (mononuclear cell-condition medium,MNC-CM) 刺激U937細胞。結果顯示,黃耆、甘草、蓮子、棗、黨參熱水萃取物之單核細胞條件培養液 (MNC-CM) 在高劑量時可有效抑制U937細胞之生長 (>40%) ,但其乙醇萃取物之單核細胞條件培養液效果則較熱水萃取物差。而不論熱水或乙醇萃取物均無直接抑制U937細胞分化之作用。在NBT還原試驗中,蓮子熱水萃取物經酒精沉澱後所得之酒精沈澱物 (100-400μg/mL) 之還原力隨著添加濃度的增加而有增加的趨勢 (31-49%) ,顯示蓮子熱水萃取之酒精沉澱物的條件培養液有明顯誘導、分化U937細胞成熟的能力。 |
英文摘要 | Effects of boiling-water extracts and 80% (v/v) ethanol extracts from Chinese medicinal herbs on the antiproliferation and differentiation of human leukemic U937 cells were examined. Conditioned medium from peripheral blood monocytes (MNC-CM) stimulated with various levels (200-800 mg/mL) and types of herb extracts (MNC-CM mode) were used to screen the effective herbs. Results showed that among the herbs tested, MNC-CM from boiling-water extracts of Huang Gi (HG), Gan Cao (GC), Lian Zi (LZ), Da Zao (DZ), and Tang Shen (TS) showed remarkable (> 40%) growth inhibition on the U937 cells at high dosage, whereas mixtures of boiling-water extracts of tested herbs with culturing medium displayed insignificant results. Meanwhile, inferior inhibition on cell proliferation was observed when U937 cells were treated with ethanol extracts with MNC-CM or conventional mode. Assays for cytoplasmic superoxide production (NBT reducing ability) by U937 cells induced by ethanol precipitates of boiling-water extracts (100-400 mg/mL) from LZ indicated a dose-dependent positive percentage (31-49%), revealing the apparent differentiation-inducing effect of MNC-CM on U937 cells. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。