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題 名 | The Intracellular Buffering Power in the Guinea-Pig Ventricular Myocyte=天竺鼠心室肌細胞內酸鹼緩衝能力之研究 |
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作 者 | 羅時鴻; 蔡建松; 李芳艷; 李國楨; 金忠孝; 鄭志鴻; 林正一; | 書刊名 | 醫學研究 |
卷 期 | 21:3 2001.06[民90.06] |
頁 次 | 頁143-158 |
分類號 | 364.6 |
關鍵詞 | 顯微螢光技術; 螢光染劑; 細胞內酸鹼值; 細胞內酸鹼緩衝能力; 心室肌細胞; Microspectrofluorimetry; Fluorescent; Intracellular pH; Intracellular buffering power; Ventricular myocyte; |
語 文 | 英文(English) |
中文摘要 | 細胞內的酸鹼調控系統是維持細胞恒定系統中的重要系統之一。細胞內總酸鹼緩衝能力(β□)可以對抗細胞內酸鹼的變化,在有關研究細胞膜上酸鹼調控的研究而言,得到明確、 精準的細胞內β□數值是不可或缺的。細胞內β□主要是包含兩個成份:內在緩衝能力(β[93bc])及二氧化碳/碳酸引起的緩衝能力(β□₂)。但是截至目前為止,很少有關於哺乳動物心室細胞內酸鹼緩衝能力的定量研究之相關報導,特別是β□₂方面的探討。所以本篇研究的主要目的就是在於定性天竺鼠單一心室肌細胞的β[93bc]及β□₂。天竺鼠單一心室肌細胞乃是利用酵素分解法獲得。細胞內的酸鹼變化大小乃是利用所謂的顯微螢光技術(microspectrofluorimetry)來偵測。使用的細胞內螢光染劑是雙發射波長的carboxy-SNARF-1。在本研究中,我們發現二氧化碳/碳酸-依賴性的細胞內酸鹼緩衝能力符合所謂二氧化碳開放系統(open system)之模式,換一句話說,β□₂的數值大小等於2.3×[HCO₃-][93bc]。這個結果和其他實驗室在羊Purkinje fiber上所得到的結果吻合。在本研究中,我們也發現無論是利用Na-acetate prepulse或NH₄Cl prepulse技術來偵測β[93bc]都可以得到相同的實驗結果,証明這兩種方法都可以被使用來偵測細胞內的緩衝能力。 |
英文摘要 | Intracellular pH (pH[93bc]) regulation is a major homeostatic system within the cell. Given the considerable role of intracellular total buffers (β□) in minimizing pH[93bc] changes, the quantification of intracellular buffering power is essential for calculating sarcolemmal acid-equivalent fluxes from pH[93bc]-recordings. Theβ□ has two components: the intrinsic buffering power of the cell (β[93bc]) and the buffering capacity caused by intracellular CO₂/HCO₃¯ (β□₂). However, thus far, the relative information in the mammalian ventricular myocyte has not been reported, especiallyβ□₂. Therefore, the aims of the present study are to determine the estimateβ[93bc] andβ□₂ in the ventricular myocyte isolated from guinea pig. pH[93bc] was measured by microspectrofluorimetry in enzymically isolated ventricular myocytes of guinea-pig. The intracellular fluoroprobe used was the dual emission carboxy-SNARF-1. We have found that the CO₂-dependent buffering in the guinea-pig ventricular myocyte is consistent with a fully open cell-system for CO₂ , i.e. β□₂ is equal to 2.3 × [HCO₃-][93bc]. This result is in agreement with previous studies in the sheep Purkinje fiber. Also, our present estimates ofβ[93bc], using either the Na-acetate or NH₄Cl prepulsing technique, are in good agreement in the guinea-pig ventricular myocyte, which suggests that the two methods give reliable and consistent measurements of the buffering power. |
本系統中英文摘要資訊取自各篇刊載內容。