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頁籤選單縮合
題名 | 油桃早期胚培養之研究=Factors Affecting Embryos Culture of Nectarine |
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作 者 | 溫英杰; 吳雅婷; | 書刊名 | 中華農業研究 |
卷期 | 49:4 2000.12[民89.12] |
頁次 | 頁48-55 |
分類號 | 435.324 |
關鍵詞 | 油桃; 胚培養; 早熟; Nectarine; Embryo culture; Early ripen; |
語文 | 中文(Chinese) |
中文摘要 | 為了育成早熟油桃(Prunus persica var. necturina Maxim.)品種,以避開低海拔地區果實蠅高峰期,本計畫以具有果實發育日數短的桃及油桃為育種親本,並用胚培養方式培養在自然狀態下無法萌芽生長的未成熟胚。試驗結果顯示,特早熟油桃的胚在果實軟化時呈嚴重退化,胚培養的適當時期為果實硬熟期。以MS培養基培養早熟和特早熟桃及油桃的雜交胚獲得74%的成苗率,較培養在NN培養基的13%及SH培養基的10%為佳。添加1mg/1BA + 1mg/1 IAA與1mg/1BA +1mg/1IBA較單獨添加或其他生長調節物質之組合為佳,但與對照比較並無顯著差異。未經低溫處理的雜交胚,萌芽率低且芽體呈簇生狀,無法發育成正常小苗。而經4℃的低溫處理的雜交胚,1∼2個月後即陸續長出胚根,可發育成正常小苗。經健化處理出瓶後成活率仍低,為尚待解決之問題。 |
英文摘要 | For breeding early-ripen nectarine varieties in order to avoid fruit fly damage in Taiwan lowland area began from May. This program chose peach and nectarine varieties which had short fruit development period and low chilling requirement as female parent and used embryo resue technique to culture immature hybrid embryos. Embryo aborted when 'Armking' and 'Armking Precoce' fruits became soft. The optimum time for culturing embryos was when fruits were firm ripe. The best results came from embryos were cultured on Murashige and Skoog medium better than those on Nitsch and Nitsch or on Steward and Hsu medium. Supplement with 1 mg/l BA + 1mg/l IAA or 1mg/BA + 1mg/l IBA on MS basal medium was better than supplement with any single plant growth regulators or combinations. However, no significant effect between MS medium with or without plant growth regulators. Embryos germinate difficulty without 4℃ treatment and usually produced plants that rosette and are difficult to grow. Transplanting the growing embryo from the culture tube to soil is the most critical stages for successful embryo culture in this program. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。