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題名 | Simultaneous Determination of Five Constituents in "Tzyy-Yun-Gau" Medicine by High Performance Liquid Chromatography=紫雲膏製劑中多成分同時分析研究 |
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作者 | 賴宏亮; 石宜仁; 葉志河; 林傳福; 梁建文; | 書刊名 | 藥物食品分析 |
卷期 | 8:4 2000.12[民89.12] |
頁次 | 頁304-308 |
分類號 | 414.33 |
關鍵詞 | 紫雲膏; 紫草; 當歸; Tzyy-Yun-Gau; Arnebiae radix; Angelicae radix; Shikonin; Deoxyshikonin; β; β-dimethylacrylshikonin; Acetylshikonin; Ferulic acid; |
語文 | 英文(English) |
中文摘要 | 使用HPLC進行傳統中藥軟膏製劑紫雲膏之成分分析,開發出包含紫草中shikonin, deoxyshikonin, β,β-dimethylacrylshikonin, acetylshikonin及當歸中ferulic acid等五種指標成分之多成分同時定量分析方法。 紫雲膏經採用n-hexane 及methanol 二種溶媒,進行分配並取methanol 層為HPLC分析之對象。將製備之試料通過保持在30 ℃恒溫之HPLC層析管(Inertsil ODS-2, 4.6 mm I.D. × 250 mm),移動相採用methanol,acetonitrile 及2% acetic acid 之混合溶液,進行梯度沖提法,以1.0 mL /分之流速沖提。五種指標成分之檢測使用UV檢出器,檢測波長設定0 . 25 分為325 nm; 25 . 58.5 分為525 nm; 58.5. 62 分為440 nm;最後62. 80分為525 nm。這個分離法對於紫雲膏中五種指標成分是精確且值得使用之定量法。 |
英文摘要 | An HPLC method was applied for the simultaneous determination of five marker substances in the traditional Chinese medicine preparation “Tzyy-Yun-Gau”. These substances included shikonin, deoxyshikonin, β,β-dimethylacrylshikonin, and acetylshikonin in Arnebiae Radix; and ferulic acid in Angelicae Radix. Tzyy-Yun-Gau was partitioned in a mixture of n-hexane and methanol, and a fraction taken into the methanol layer was analyzed. The samples were run through an HPLC column (Inertsil ODS-2, 4.6 mm I.D. × 250 mm) at 30°C with a mobile phase consisting of methanol, acetonitrile and 2% acetic acid with linear gradient elution at a flow-rate of 1.0 mL/min. A UV detector was used for detection. The detection wavelength varied with time, which was 325 nm during 0~25 min, 525 nm during 25~58.5 min, 440 nm during 58.5~62 min, and 525 nm during 62~80 min. This could be a successful separation method for the simultaneous determination of five marker substances in “Tzyy-Yun-Gau”. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。