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| 題 名 | Effects of Crude Drugs on Glucose Uptake in 3T3-L1 Adipocytes=中藥對3T3-L1脂肪細胞糖分攝取的影響 |
|---|---|
| 作 者 | 洪秀貞; 馮濟敏; 黃佳惠; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
| 卷 期 | 16:9 2000.09[民89.09] |
| 頁 次 | 頁445-451 |
| 分類號 | 414.5 |
| 關鍵詞 | 中藥; 3T3-L1脂肪細胞; 糖分; 攝取; 2-deoxyglucose uptake; 3T3-L1 adipocytes; Crude drugs; |
| 語 文 | 英文(English) |
| 中文摘要 | 本實驗主要是探討六種中藥用水抽出的成份對3T3-L1脂肪細胞之 3H-2-deoxyglucose糖分攝取的影響。這六種中藥包括黃耆、人參、黃芩、地骨 皮、黃柏及麥門冬。結果發現3T3-L1脂肪細胞經過不同濃度藥物處理60分鐘後， 糖分的攝取由控制組的140 pmoe/min/mg protein呈現不同的變化。經黃耆處理後 細胞糖分的攝取量由517 (0.1 mg/ml)，201 (1 mg/ml) 降到97 (10 mg/ml)，人參對 糖分攝取量則由324 (0.1 mg/ml)，146 (1 mg/ml)降到46 (10 mg/ml)，黃芩對糖分 攝取量由215 (0.1 mg/ml)，213 (1 mg/ml)降到34 (10 mg/ml)，地骨皮對糖分攝取 量由230 (0.1 mg/ml)，188 (1 mg/ml)降到38 (10 mg/ml)，黃柏對糖分攝取量由142 (0.1 mg/ml)，132 (1 mg/ml)降到24 (10 mg/ml)，麥門冬對糖分攝取量由489 (0.1 mg/ml)，374 (1 mg/ml)升到344 (10 mg/ml)。細胞經過胰島素處理後，糖分攝取 量則增加到570 pmole/min/mg protein，經黃耆處理後，糖分攝取量由816 (0.1 mg/ml)，674 (1 mg/ml)到532 (10 mg/ml)，人參對糖分攝取量由254(0.1 mg/ml)， 123 (1 mg/ml)降到76 (10 mg/ml)，黃芩對糖分攝取量由315 (0.1 mg/ml)，265 (1 mg/ml)降到33 (10 mg/ml)，地骨皮對糖分攝取量由281 (0.1mg/ml)，248 (1 mg/ml) 降到37 (10 mg/ml)，黃柏對糖分攝取量由747 (0.1 mg/ml)，523 (1 mg/ml)降到 33(10 mg/ml)，麥門冬對糖分攝取量由 753 (0.1 mg/ml)，740 (1 mg/ml)到421 (10 mg/ml)。由以上結果顯示不論是在缺乏或有胰島素的存在狀況下，適當濃度的黃 耆及麥門冬水溶性的抽出物都會增加3T3-L1脂肪細胞對糖分的攝取量。 |
| 英文摘要 | In this study, various water-extracted crude drugs from Radix Asparagi, Radix Ginseng, Radix Scutellariae, Cortex Lycii Radicis, Cortex Phellodendri and Radix Ophiopogonis were investigated in their effects on [3H]-2-deoxyglucose uptake in 3T3-L1 adipocytes. Following treatment of cells with various crude drugs for 60 mim, the basal [3H]-2-deoxyglucose uptake in cultured 3T3-L1 cells was changed by Radix Asparagi from 140 pmole/min/mg protein of control to 513 (0.1 mg/ml), 201(1 mg/ml) and 97 (10 mg/ml). Glucose uptake was changed to 324 (0.1 mg/ml), 146 (1 mg/ml) and 46 (10 mg/ml) with Radix Ginseng. In the presence of Radix Scutellariae, glucose uptake was changed to 215 (0.1 mg/ml), 213 (1 mg/ml) and 34 (10 mg/ml). In the presence of Cortex Lycii Radicis, glucose uptake was 230 (0.1 mg/ml), 188 (1 mg/ml) and 38 (10 mg/ml). In the case of Cortex Phellodendri and Radix Ophiopogonis, uptake was changed to 142 (0.1 mg/ml), 132 (1 mg/ml), 24 (10 mg/ml) and 489 (0.1 mg/ml), 374 (1 mg/ml), 344 (10 mg/ml), respectively. In insulin-stimulated cells, the [3H]-2-deoxyglucose uptake was changed by Radix Asparagi from 570 pmole/min/mg protein of the control to 816 (0.1 mg/ml), 674 (1 mg/ml) and 532 (10 mg/ml). After incubation with Radix Ginseng, the glucose uptake was changed to 254 (0.1 mg/mi), 123 (1 mg/mi) to 76 (10 mg/mi). In the presence of Radix Scutellariae, the glucose uptake was changed to 315 (0.1 mg/ml), 265 (1 mg/ml) and 33 (10 mg/ml). After incubation of Cortex Lycii Radicis, the uptake activity was changed to 281(0.1 mg/ml), 248 (1 mg/ml) and 37 (10 mg/ml). In the case of Cortex Phellodendri and Radix Ophiopogonis, the activity of glucose uptake was measured as 747 (0.1 mg/ml), 523 (1 mg/ml), 33 (10 mg/ml) and 753 (0.1 mg/ml), 740 (1 mg/ml), and 421 (10 mg/ml), respectively. These results indicate that the water-extracted materials of Radix Asparagi and Radix Ophiopogonis increase the glucose uptake in basal and insulin-stimulated 3T3-L 1 adipocytes. |
本系統中英文摘要資訊取自各篇刊載內容。