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頁籤選單縮合
題名 | Culture-dependent Variation in Esterase Isozymes and Malathion Susceptibility of Diamondback Moth, Plutella Xylostella L.=小菜蛾不同品系其酯解異構酶及馬拉松抗藥性之相關性的差異研究 |
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作者姓名(中文) | 馬堪津; 廖信昌; | 書刊名 | 動物研究學刊 |
卷期 | 39:4 2000.10[民89.10] |
頁次 | 頁375-386 |
分類號 | 433.3 |
關鍵詞 | 小菜蛾; 酯解酶; 抗藥性; 相互關聯; Plutella xylostella L.; Esterase isozymes; Resistance; Correlation; |
語文 | 英文(English) |
中文摘要 | 吾人以單雌雄交配法,成功地由社子菜蛾族群中分離出五個對馬拉松具不同抗藥性的品系。就幼蟲半致死劑量最高的社子十號而言,其抗藥性是感藥品系社子十五號的二十九倍、是十二號的二十二倍:抗性次高的社子二十六號及三十四號則為十二號的十三倍。如以羽化成功率之半致死劑量來估算,則十號為十二號的九百二十倍。協力劑毒效試驗結果證實社子二十六號者其抗馬拉松特性和酯解[]及谷胱[][]硫轉移[]有關。從乙酸酯為受質去染由電泳分離之酯解[]譜,可看出十、二十六、三十四號品系的族群體內均含高頻率的第八乙及九乙號酯解[]譜,此正是十二號者所缺。反之十二號有的八及九號酯解[],在抗藥族群身上也不易找到。社子十五及十號在抗藥性及[]譜的穩定度上無法掌控。社子二十六及三十四號兩品系,在抗藥性之特徵上,在酯解[]譜上皆具類似的表現,兩者所異在於快速游動居陰極區的酯解[]。以社子二十六號所生後代作單雌自交,得到含第八乙及九乙酯解[]發生頻率高低的各亞品系。各亞品系所具抗馬拉松的強弱與其所異之第八乙及九乙酯解[]發生頻率高 低呈統計上的正相關。如將社子十二號的亞品系所生後代作單雌自交,可在其第二代測得第三代酯解[]發生頻率之高低,與其低抗藥性之強弱呈正相關;第三代可測得第四號酯解[]頻率之高低,卻與弱抗藥性之強弱呈負相關。諸由異構酯解[]發生頻率與抗藥性的相關性間呈現的生命意義,其將在本文討論,如何利用具隱性基因調控的第四乙及九乙號酯解[],以作檢測田間小菜蛾族群抗馬拉松的可能性,亦一併討論之。 |
英文摘要 | Five cultures of the Sheh-Tzu diamondback moth, Plutella xylostella L., showing different levels of malathion resistance, were successfully raised by the single male-female mating method. The LD50 of malathion to ST10 was 29- and 22-fold that to ST15 and ST12, respectively. ST26 and ST34 possessed 14- and 13-fold the resistance to malathion as did ST12. The difference in the adult emergence rate of treated larvae between ST10 and ST12 was 920 fold. Toxicity of malathion to ST26 larvae was mostly synergistically enhanced by S, S-tributyl phosphortrithioate and diethyl maleate. A zymogram study using 1-naphthyl acetate as a substrate on PAGE gel showed that ST10, 26, and 34 were possessed of significantly higher frequencies of esterases 8b/9b which were rarely found in ST12 Larvae. Esterases 9 and 8null were found in most susceptible larvae. ST26 shared toxicological characteristics against malathion and in biochemical properties of the slow-moving esterase with ST34. Subcultures of ST26, which showed differential susceptibility to malathion, were significantly and linearly correlated with the differential frequency of esterases 8b/9b of the moth. Frequencies of esterases 3 and 4b of subcultures were either positively or negatively correlated with a low titer of resistance. The significance of the correlation between isozyme frequency and malathion resistance of DBM is discussed. The possibility of using the recessive alleles of esterases 4b and 9b as indicator proteins for monitoring malathion resistance of the DBM is also discussed. |
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