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題 名 | Enhanced Nuclease Production from a Recombinant "Escherichia coli" by Magnesium Salts=添加鎂鹽以促進基因重組大腸桿菌生產核酸分解酵素 |
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作 者 | 林晉平; 王大榮; 陳特良; 吳昭燕; 何漣漪; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷 期 | 33:2 2002.03[民91.03] |
頁 次 | 頁175-180 |
分類號 | 460.02 |
關鍵詞 | 鎂鹽; 基因重組; 大腸桿菌; 核酸分解酵素; Nuclease fermentation; Magnesium ion; Vibrio vulnificus; Recombinant escherichia coli; |
語 文 | 英文(English) |
中文摘要 | 本文探討添加鎂鹽對以基因重組大腸桿菌,生產源自於創傷弧菌的一種具熱穩定性的核酸分解酵素之促進效應。研究發現,在醱酵液中添加適當量的鎂鹽,對酵素的生產是相當重要的。在LB培養基中培養此基因重組菌。可得核酸分解酵素產量62U/mL。添加50mM MgC□至LB培養基中,可增加酵素產量至380U/mL。增加細胞濃度可以促進酵素之生產,但是,其前提是培養基中需存在有足夠量的鎂離子。然而,鎂鹽的用量過多反而對酵素之生產不利,其中,鎂鹽的種類是一個影響因素。在LB培養基中添加額外的碳源、氮源、和適當濃度的MgS□,可使核酸分解酵素的產量達3750U/mL。 |
英文摘要 | The enhancement by means of magnesium salt of the production of a thermal stable muclease of Vibrio vulnificus with a recombinant Escherichia coli was investigated. Supplementation of the medium with a proper amount of magnesium salt was found to be critical for enzyme production. Cultivation of the recombinant strain in LB medium resulted in a nuclease yield of 62 U/mL. Supplementation of the LB medium with 50 mM MgC□ increased the yield to 380 U/mL. Increasing the cell concentration could enhance the nuclease yield provided that a sufficient amount of magnesium ion was present in the medium. However, an overdose of magnesium salt had an adverse effect on enzyme production, where the type of magnesium salt was an influential factor. A nuclease yield of 3750 U/mL was achieved by supplementing LB medium with additional carbon and nitrogen sources, and with a proper concentration of MgS□. |
本系統中英文摘要資訊取自各篇刊載內容。