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題名 | 膨脹床吸附技術應用在酵素的直接回收程序:以α-配醣酵素為例=Application of Expanded Bed Adsorption Technology for Direct Recovery Process of Enzymes: A Case Study of α-Glucosidase |
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作者 | 蘇家弘; 葉昭賢; 張煜光; | 書刊名 | 明志技術學院學報 |
卷期 | 32 2000.06[民89.06] |
頁次 | 頁105-116 |
分類號 | 463.824 |
關鍵詞 | 麵包酵母菌; α-配醣酵素; 膨脹床吸附技術; STREAMLINE DEAE; STREAMLINE phenyl; Baker's yeast; α-glucosidase; Expanded bed adsorption technology; |
語文 | 中文(Chinese) |
中文摘要 | 本研究係發展一種新的酵素純化技術-即膨脹床吸附技術,從未澄清麵包酵母菌 研磨液中,有效的直接回收α - 配醣酵素。 選用 STREAMLINE 離子交換型與疏水作用力型 樹脂作為膨脹床吸附程序的吸附劑。在一系列小規模批式與填充床的試驗,以尋找適當操作 條件作為提升純化效果的依據,實驗內容包括吸附 pH 值的選擇與離子強度對吸附程序的影 響,並探討其動相鍵結量與沖提條件的選擇,來比較離子交換型與疏水型樹脂對酵素純化的 效果。 結果顯示,二種樹脂對α - 配醣酵素活性的回收率約 80 %,而純度提升為二倍左 右。 對產能而言,離子交換型的操作模式高於疏水作用力型樹脂,因此,STREAMLINE 離子 交換樹脂更適合應用於大規模的純化程序。 |
英文摘要 | A novel enzyme purification technology, expanded bed adsorption (EBA), has been developed for efficient direct recovery of α -glucosidase from unclarified baker's yeast cell homogenate. STREAMLINE ion exchange and hydrophobic interaction adsorbents were chosen for the EBA purification process. For improving purification efficiency, optimal operating conditions were determined in a series of small scale batch and packed bed experiments. These experiments included the choice of adsorption pH, the effect of ionic strength for the adsorption process, the study of dynamic binding capacity, and the choice of elution conditions. Ion exchange and hydrophobic interaction adsorbents were compared for their purification performance. The results showed that both adsorbents for the direct recovery of activity of α -glucosidase by using expanded bed chromatography were approximately 80%. However, the purification factor was only two-fold. On the basis of these results, the relative productivity of ion exchange operating mode was higher than that of hydrophobic interaction operating mode. Hence, STREAMLINE ion exchange adsorbent is more suitable for use in large scale purification processes. |
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