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題 名 | Construction and Characterization of a recA Mutant of Xanthomonas Campestris pv. Citri=柑橘潰瘍病菌recA突變株之建立與特性分析 |
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作 者 | 楊源昌; 楊美桂; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 41:2 2000.04[民89.04] |
頁 次 | 頁129-137 |
分類號 | 435.33 |
關鍵詞 | 基因重組; 互補; DNA修補; Complementation; DNA repair; Homologous recombination; RecA; |
語 文 | 英文(English) |
中文摘要 | 自柑橘潰瘍病菌 (Xanthomonas campestris pv. citri) 所選殖之 recA 基因,是由 1032 個核甘酸所組 成,可合成含 344 個氨基酸之蛋白質。其序列與其他細菌之 recA 基因很類似,合成之蛋白在大腸桿菌內 亦具有功能。利用插入性突變法,得到之 recA 突變株,對 DNA 突變劑極為敏感,也失去基因重組的能 力。選殖之 recA 基因,可形成一 37 kDa 之蛋白,可與抗大腸桿菌 RecA 之抗體作用。另外,在 recA 基因之上游,還發現一可產生 213 氨基酸之蛋白的基因,相似於大腸桿菌之 lexA 基因;而在其下游,還有 一由 153 氨基酸所組成之蛋白之基因,相當於綠膿桿菌之 recX 基因。 |
英文摘要 | The recA gene of Xanthomonas campestris pathovar citri (X. c. pv. citri) was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame of 1,032 bp that encodes a 344-amino acid protein and which shows a high degree of homology to recA genes from other bacteria. The cloned recA gene of X. c. pv. citri restored to a recA deletion mutant of Escherichia coli the ability to resist killing induced by methylmethane sulfonate or ultraviolet radiation, indicating that the cloned gene was functional in E. coli. A recA mutant of X. c. pv. citri was constructed by gene replacement and was shown both not to possess detectable DNA recombination activity and to be markedly more sensitive to DNA-damaging agents than is wild-type X. c. pv. citri. Transformation of recA mutants of E. coli and X. c. pv. citri with a plasmid containing X. c. pv. citri recA conferred the ability to produce a 37-kDa protein that reacted with antiserum to E. coli RecA protein. Two additional open reading frames were also identified in the recA region of the X. c. pv. citri genome: One located immediately upstream from recA that encodes a 213-amino acid protein with a high degree of similarity to the LexA protein of E. coli, and another located immediately downstream of recA that encodes a 153-amino acid protein with sequence similarity to the RecX protein of Pseudomonas aeruginosa. |
本系統中英文摘要資訊取自各篇刊載內容。