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題 名 | 三種農用藥劑對小鼠精子活力、體外受精率及胚胎發育之影響=The Effects of Three Pesticides on Sperm Motility, in Vitro Fertilization and Embryo Development in Mice |
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作 者 | 呂水淵; 王順成; | 書刊名 | 中華民國獸醫學會雜誌 |
卷 期 | 26:1 2000.03[民89.03] |
頁 次 | 頁24-35 |
分類號 | 433.85 |
關鍵詞 | 小鼠; 體外受精; 生殖毒性; Mouse; In vitro fertilization; Reproductive toxicity; |
語 文 | 中文(Chinese) |
中文摘要 | 本試驗利用具致胚胎畸形性藥劑免賴得(benomyl)、無致胚胎畸形性之甲基多保淨(thiophanatemethyl)及具生殖毒性之Aroclor-1254藥劑試對小鼠精子活力及對精子、卵子之生體外受精率及胚發育之影響,期建立生殖毒性藥劑活體外測試之部分模式。結果顯示,當精子與卵子同時以免賴得與甲基多保淨處理,僅在最高濃度(100 μg/mL)時顯著降低小鼠受精率,而Aroclor-1254則隨濃度增加而降低受精率。若卵子先以藥劑處理6小時而精子不處理時,甲基多保淨並不影響受精率,而免賴得與Aroclor-1254則在中、高濃度(≧10 μg/mL)均顯著降低受精率(P<0.05)。若精子先處理1.5小時而卵子不處理時,甲基多保淨亦不影響受精率,但免賴得與Aroclor-1254則在最高濃度(100 μg/mL)時降低受精率(P<0.05)。至於在精子活動力方面,暴露初期(2小時)之精子活動力均較對照組低,但無濃度-反應相關; 處理8和10小時後,精子之活動力均顯著低於對照組(P<0.05),且呈濃度-反應關係。胚發育影響上,三藥劑處理48小時後,高濃度均顯著影響胚發育;處理96小時後,則於低濃度即可影響胚之發育。綜合上述結果,甲基多保淨、免賴得及Aroclor-1254等三藥劑均會影響小鼠之生體外受精率及胚之發育,其影響程度以免賴得與Aroclor-1254為較嚴重,而甲基多保淨則較輕微。 |
英文摘要 | This study on reproductive toxicity was conducted utilizing teratogen, benomyl, nonteratogen, thiophanate-methyl in vivo test, and positive reproductive toxicity agent of Aroclor-1254 to investigate end point sperm motility, in vitro fertilization after 6-hour ovum-exposure was not influenced, to thiophanate-methyl while that to benomyl and Aroclor-1254 in midhigh doses was reduced (P>0.05). In vitro fertilization after 2-hour sperm-exposure to thiophanate-methyl was comparable to that of the control while that to benomyl and Aroclor-1254 in high dose was decreased. Sperm motility showed no dose-response relationship though it was not comparable to that of the control during the 2-hour exposure. Sperm motility during the 8-and 10-hour exposure to the three chemicals were more significantly (P<0.05) decreased than that of the control. Embryo development was influenced by the three chemicals in high doses during the 48-hour exposure while it was significantly (P<0.05) reduced by those in low doses during the 96-hour exposure. To sum up, there were different degrees of influence on sperm motility, in vitro fertilization and embryo development. The effect of benomyl and Aroclor-1254 on these parameters were larger than that of thiophanate-methyl. |
本系統中英文摘要資訊取自各篇刊載內容。