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題名 | 免疫電子顯微鏡技術導論--原理、方法暨皮膚科學之應用=Introduction to Immune Electron Microscopy--Principles, Methods and Applications in Dermatology |
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作者姓名(中文) | 陳文杰; | 書刊名 | 中華皮膚科醫學雜誌 |
卷期 | 17:4 1999.12[民88.12] |
頁次 | 頁282-299 |
分類號 | 415.7 |
關鍵詞 | 免疫電子顯微鏡; 皮膚科學; 皮膚水兊病; Immune electron microscopy; Skin basement membrane zone; Autoimmune subepidermal bullous dermatoses; |
語文 | 中文(Chinese) |
中文摘要 | 免疫電子顯微鏡的方法由SJ Singer於一九五九年首度發表,一九七0年代成功地 應用在皮膚水�秤f之研究,證實了尋常性天�紗H及類天�紗H的抗原位置不同。近二十年來由 於新的標計物質(如微小至直徑 10mm 以下的膠質金原子顆粒), 新的包埋物質(如 LR White, Lowicry1 K4M, K11M 等)以及冷凍顯微切片技術的發展,使得免疫電子顯微鏡成為 研究及診斷皮膚疾患之重要工具,尤其對了解表皮細胞橋體及皮膚基底膜區帶的顯微結構, 以及各種水�祟吤眽l及其相關抗原的分析有很大的貢獻。本文前半部將討論免疫電子顯微鐘 技術的基本原理、方法步驟及最近之發展,包括前包埋及後包埋免疫電子顯微鏡方法與應用 時機之比較。後半部則將描述皮膚基底膜區帶的分子結構及相關之自體免疫性表皮下水�砲� 疹,藉以介紹免疫電子顯微鏡技術在臨床皮膚疾病診斷之應用。 |
英文摘要 | Immune electron microscopy (IEM), first described by SJ Singer in 1959, has been successfully used in 1970s to localize different antigens specific to pemphigus vulgaris and bullous pemphigoid, respectively. Over the past two decades, development of new immunolabels (LR White, Lowicry1 K4M, K11M, etc), and cryoultramicrotomy has made IEM among the most important tools for the study and diagnosis of skin diseases, especially for our better understanding of the ultra-structures of epidermal desmosome and skin basement membrane zone, their related bullous dermatoses as well as the corresponding tissue antigens. Here we discuss first the principles, methods/procedures and recent advancement of IEM, including the differences between pre-embedding vs. post-embedding IEM and their suitable indications. The application and use of IEM in the clinical investigation of some autoimmune subepidermal bullous dermatoses are then presented, with emphasis on the recent findings of the molecular structures of skin basement membrane zone. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。