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題名 | 報歲蘭利用根莖(rhizome)組織繁殖種苗之研究=Studies on Utilization of Rhizome for Rapid Propagation of Cymbidium Sinense Willd |
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作 者 | 許文章; 蔡智賢; 廖成康; | 書刊名 | 嘉義技術學院學報 |
卷期 | 67 1999.12[民88.12] |
頁次 | 頁1-12 |
分類號 | 434.28 |
關鍵詞 | 報歲蘭; 根莖; 葉芽; 培養基; Cymbidium sinense willd; Rhizome; Shoot; Medium; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗以報歲蘭種子無菌播種之根莖為材料,探討報歲蘭種苗大量繁殖之方法。結果顯示以1/2 MS巨量元素加全量微量元素、維他命、20 g/L蔗糖之培養基為基礎培養基,添加3 g/L活性碳可以明顯促進歲蘭根莖之重量;於基礎培養基中再添加0.1 mg/L BA及有機物,可促進根莖重達7.55 g,葉芽之重量亦達6.31 g。基礎培養基中添加活性碳不利於根莖分化成葉芽, 若再添加 0.5 mg/L NAA 及 5 mg/L BA 則有利於根莖分化成葉芽。移植至定植培養基之報歲蘭幼苗,以長度3-5 cm以上之中、大苗為宜,3 cm以下之小苗移植到定植培養基,平均有54%之苗會逆分化成根莖,不利苗株之培育。定植培養基內含有1/2 MS巨量元素、全量微量元素、維他命、活性碳、NAA、tryptone及有機添加物作為定植培養基,定植4個月苗株可達10 cm以上,其單株根數8-9根最多,根長15-16 cm最長,為一良好定植培養基。 |
英文摘要 | The purpose of this study was to explore the method of rapid propagation of Cymbidium sinense willd by using rhizomes of seed gemination in vitro. The study has shown that adding 3 g/L active charcoal to the medium, which is composed of 1/2 MS of macronutrient, whole micronutrient and vitamins, and 20g/L sucrose, would evidently promote the rhizome weight of cymbidium sinense Willd. The rhizome weight would be 7.55 g and the shoot weight would be 6.31 g if 0.1 mg/L BA and organic matters were added to the medium. Adding active charcoal was not good for shoot formation from rhizome. However, if the medium was supplemented with 0.5 mg/L NAA and 5 mg/L BA, it would be good for shoot formation. Shoots of medium or large size--about 3 to 5 cm, were ideal for transplanting. On the average, 54% of the shoots which were less than 3 cm would transform into rhizome. Thus, it was not good for the plant growth. The medium containing 1/2 MS macronutrient, whole micronutrient, vitamins, active charcoal, NAA, tryptone, and organic matters was perfect for shoot setting. After 4 months of setting, the plant would grow to more than 10cm tall. Each plant might have 8 to 9 roots, and the root length might reach from 15 to 16 cm long. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。