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題名 | 豬假性狂犬病gE醣蛋白及胸腺核苷激酶基因缺損株活毒疫苗之研發=Evaluation of Efficacy and Safety of an Attenuated Seudorabies Vaccine with Deletions in the Thymidine Kinase and Glycoprotein E Genes |
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作者 | 朱純燕; 蕭璦莉; 張清華; 張天傑; 林家修; 吳昭良; Chu, Chun-yen; Shiau, Ai-li; Chang, Ching-hwa; Chang, Tien-jye; Lin, David Chia-shyu; Wu, Chao-liang; |
期刊 | 臺灣畜牧獸醫學會會報 |
出版日期 | 19990600 |
卷期 | 69:1/2 民88.06 |
頁次 | 頁59-68 |
分類號 | 437.657 |
語文 | chi |
關鍵詞 | 假性狂犬病病毒; gE醣蛋白; 胸腺核苷激酶損; 活毒標識疫苗; Pseudorabies virus; Glycoprotein E; Thymidine kinase; Gene-deleted mutant; Live marker vaccine; |
中文摘要 | 利用重組DNA技術,由桃園豬場分離之豬假性狂犬病毒構築了一株gE 醣蛋白及胸 腺核��激�t (thymidinekinase;TK) 雙基因缺損株 (PRV-gE �� /TK �� ),作為假性狂犬病 活毒標識疫苗之種毒株。病毒生長動力學之比較顯示 PRV- gE �� /TK �狾b細胞增殖培養, 病毒力價比野生株或 gE 單基因缺損株約低 100 倍,且其所形成的病毒斑較小。 經試製威 活毒疫苗.免疫於小鼠及豬隻,所有的試驗動物均煞異常反應且增重健存; 在仔豬體內連續 繼代五代無毒力迥歸現象。 以 PRV-gE �� /TK �狳葶怳p鼠,可產生假性狂犬病毒特異性抗 體; 在細胞免疫反應方面,可刺激小鼠脾臟 T 細胞之增殖反應。 在豬隻方面,豬經兔疫後 ,可誘發產生中和性抗體,但不產生對抗 gE 之抗體。兔疫過之小鼠及豬隻,可耐過假性狂 犬病毒強毒攻擊。 因此 PRV-gE �� /TK �畟飌穧]缺損株疫苗證實是安全而有效的活毒疫苗 ,和檢測抗 gE 抗體之免疫酵素吸附試驗併用,方可作為野外感染與疫苗兔疫的區分指標。 |
英文摘要 | A pseudorabies virus (PRV) mutant with deletions in genes for glycoprotein E (gE) and thymidine kinase (TK), designated PRV-gE �� /TK ��, has been constructed from a Taiwan local strain using recombinant DNA technology and exploited as a modified live marker vaccine. A hundred-fold decrease in virus yield was observed in cells infected with PRV-gE �� /TK �� compared with those infected with wild-type or gE-deleted PRV. Likewise, plaque size produced by PRV-gE �� /TK �� was significantly smaller than that produced by the parental viruses. The efficacy and safety of the PRV-gE �� /TK �� vaccine were evaluated in mice and pigs. PRV-specific antibodies were detected in sera from mice immunized with PRV-gE �� /TK ��. Spleen cells from immunized mice also developed PRV-specific T-cell proliferative responses in vitro. Furthermore, the immunized mice were protected from lethal PRV challenge. Normal weight gain with no clinical signs was observed in pigs immunized with the PRV-gE �� /TK �� live vaccine. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Vaccination with PRV-gE �� /TK �� elicited PRV neutralizing antibodies but no anti-gE antibodies in pigs, which rendered them resistant to virulent PRV challenge. Thus, the use of the PRV-gE �� /TK �� live vaccine in combination with an ELISA to detect antibodies to gE can be used to differentiate vaccinated from naturally infected animals. The results demonstrate that the PRV-gE �� /TK �� vaccine is efficacious and safe for the control of PR disease. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。