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題 名 | Assisted Hatching Achieved with the Use of Pronase in a Mouse Model at Various Embryo Stages=以Pronase在不同胚胎時期協助老鼠胚胎孵化之效果 |
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作 者 | 蔡依□; 楊政憲; 吳明義; 盧信芬; 何弘能; 楊友仕; | 書刊名 | 中華民國婦產科醫學會會刊雜誌 |
卷 期 | 38:4 1999.12[民88.12] |
頁 次 | 頁141-146 |
分類號 | 417.131 |
關鍵詞 | 胚胎協助孵化; 透明層部份切割; Assisted hatching; Partial zonal dissection; Pronase; |
語 文 | 英文(English) |
中文摘要 | 目的:透明層溶化為胚胎孵化過程中必要前提之一。依此推論,較諸以透明層部份切割或Tyrode's溶液產生一透明層缺口,利用化學溶液使透明層變軟應可獲得更佳之協助胚胎孵化效果。 方法:本實驗中,總計有156個四細胞期及160個八細胞期之老鼠胚胎接受pronase溶液(10IU/mL)作用一、二成三分鐘,其後利用HTF溶液清洗胚胎二次以去除殘存之pronase。此外有59個四細胞期及59個八細胞期胚胎充當對照組,亦即不接受pronase作用。上述胚胎均於37℃及5% CO�素藿狺U在HTF溶液中繼續培養三至四天,以觀察胚胎發育及孵化情形。 結果:不論胚胎接受pronase作用一、二、或三分鐘,其發育及孵化的情形均極為相似,因此將所有接受pronase作用的胚胎視為同一組(實驗組)來進行下列比較。結果發現實驗組與對照組中胚胎發育至囊胚期的比率並無差別。然而不論是四細胞期或八細胞期的胚胎,接受pronase作用者完成孵化的比率明顯高於對照組(P值分別為0.029及<0.001)。 結論:老鼠胚胎接受pronase作用後可提高其孵化機率,然而人類胚胎是否可獲得此一結果仍有待進一步研究。 |
英文摘要 | OBJECTIVE: Embryo hatching requires lysis of the zona pellucida (ZP) by a trypsin-like protease in vivo. Therefore, thinning of the ZP with chemical solutions might be a preferable method of assisting embryo hatching than merely producing a zonal hole, which is accomplished by either partial zona dissection or with acid Tyrode's solution. METHODS: A total of 316 mouse embryos, 156 at the 4-cell and 160 at 8-cell stage, were cultured in pronase medium (10 IU/mL) for 1, 2, or 3 minutes. Afterwards, the embryos were washed twice with human tubal fluid (HTF) medium to remove the residual content of pronase. There were also 118 embryos, 59 at the 4-cell and 59 at the 8-cell stage, which served as controls without pronase treatment. All embryos were cultured in HTF medium at 37@C and 5% CO�� atmosphere for an additional 3-4 days to observe their development and hatching process. RESULTS: Since both the percentages of blastocyst development and embryo hatching were similar among those treated with pronase for 1, 2, and 3 minutes, the pronase-treated embryos were regarded as similar ones in the following comparison. The blastocyst developing rates did not differ between those treated with pronase and those without treatment. However, embryos treated with pronase achieved a much higher percentage in completing the hatching process than did the control ones, both at the 4-cell (P=0.029) and 8-cell stage (P<0.001). CONCLUSIONS: The addition of pronase at the 4-cell or 8-cell stage improves embryo hatching in a mouse model. However, the utilization of pronase in human embryos needs further investigation. |
本系統中英文摘要資訊取自各篇刊載內容。